Acoustic fingerprints of dye-labeled protein submicrosphere photoacoustic contrast agents

Michael A. McDonald, Ladislav Jankovic, Khalid Shahzad, Michael Burcher, King C.P. Li

Research output: Contribution to journalArticlepeer-review


Dye-labeled protein microspheres, submicron in size and capable of producing thermoelastically generated ultrasound in response to laser stimulation, are presented as contrast agents for photoacoustic imaging. Incident laser energy absorbed by fluorescein isothiocyanate (FITC)-labeled elastin submicrospheres results in thermoelastically generated sound production. Plotted A-line graphs reveal a distinctive morphology and a greater than two orders of magnitude increase in signal amplitude subsequent to converting FITC elastin into submicrospheres (despite a four orders of magnitude decrease in concentration). Evidence of nonlinearity and enhancement of ultrasound backscatter indicate a potential use in contrast-enhanced harmonic imaging. Photoacoustic and ultrasound imaging of FITC-elastin submicrospheres in a water-filled phantom vessel shows enhanced contrast at low concentration and clear delineation of the phantom vessel wall.

Original languageEnglish (US)
Article number034032
JournalJournal of biomedical optics
Issue number3
StatePublished - 2009
Externally publishedYes


  • contrast agents
  • photoacoustic
  • protein microspheres
  • ultrasound

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering


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