ACL5: An Arabidopsis gene required for internodal elongation after flowering

Yoshie Hanzawa, Taku Takahashi, Yoshibumi Komeda

Research output: Contribution to journalArticle

Abstract

In rosette plants, the formation of initial flowers is closely linked to the lengthening of internodes (bolting). In order to clarify the molecular basis of bolting, mutants with reduced lengths of internodes were screened. This paper presents the identification and characterization of recessive mutations in ACAULIS5 (ACL5), a gene required for internodal growth in Arabidopsis thaliana. Unlike previously described mutants with reduced size of organs, the acl5 mutant has a severe defect that is restricted to the process of cell elongation after transition to the reproductive stage and shows no phenotype before floral induction. The results of RNA blot hybridizations showed that the acl5 mutation causes a striking reduction in the transcript levels of genes encoding the tonoplast intrinsic protein (γ-TIP) and the endoxyloglucan transferase (EXGT-A1), both of which have recently been suggested to be important for cell elongation. Furthermore, our morphological study indicates that the mutation also causes proliferative arrest of the apical inflorescence meristem. These results strongly suggest that, during the reproductive phase, the wild-type ACL5 gene product has a critical function not only in the control of elongation growth of organs but also in the continued maintenance of the proliferative activity of flower-producing meristems.

Original languageEnglish (US)
Pages (from-to)863-874
Number of pages12
JournalPlant Journal
Volume12
Issue number4
DOIs
StatePublished - Jan 1 1997

Fingerprint

Arabidopsis
bolting
Meristem
flowering
mutation
meristems
internodes
mutants
Mutation
cell growth
Genes
flowers
Rosette Formation
Inflorescence
genes
Organ Size
tonoplast
Growth
transferases
hybridization

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

ACL5 : An Arabidopsis gene required for internodal elongation after flowering. / Hanzawa, Yoshie; Takahashi, Taku; Komeda, Yoshibumi.

In: Plant Journal, Vol. 12, No. 4, 01.01.1997, p. 863-874.

Research output: Contribution to journalArticle

Hanzawa, Yoshie ; Takahashi, Taku ; Komeda, Yoshibumi. / ACL5 : An Arabidopsis gene required for internodal elongation after flowering. In: Plant Journal. 1997 ; Vol. 12, No. 4. pp. 863-874.
@article{9cd3a0ef25fe4f829841bea796034e73,
title = "ACL5: An Arabidopsis gene required for internodal elongation after flowering",
abstract = "In rosette plants, the formation of initial flowers is closely linked to the lengthening of internodes (bolting). In order to clarify the molecular basis of bolting, mutants with reduced lengths of internodes were screened. This paper presents the identification and characterization of recessive mutations in ACAULIS5 (ACL5), a gene required for internodal growth in Arabidopsis thaliana. Unlike previously described mutants with reduced size of organs, the acl5 mutant has a severe defect that is restricted to the process of cell elongation after transition to the reproductive stage and shows no phenotype before floral induction. The results of RNA blot hybridizations showed that the acl5 mutation causes a striking reduction in the transcript levels of genes encoding the tonoplast intrinsic protein (γ-TIP) and the endoxyloglucan transferase (EXGT-A1), both of which have recently been suggested to be important for cell elongation. Furthermore, our morphological study indicates that the mutation also causes proliferative arrest of the apical inflorescence meristem. These results strongly suggest that, during the reproductive phase, the wild-type ACL5 gene product has a critical function not only in the control of elongation growth of organs but also in the continued maintenance of the proliferative activity of flower-producing meristems.",
author = "Yoshie Hanzawa and Taku Takahashi and Yoshibumi Komeda",
year = "1997",
month = "1",
day = "1",
doi = "10.1046/j.1365-313X.1997.12040863.x",
language = "English (US)",
volume = "12",
pages = "863--874",
journal = "Plant Journal",
issn = "0960-7412",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - ACL5

T2 - An Arabidopsis gene required for internodal elongation after flowering

AU - Hanzawa, Yoshie

AU - Takahashi, Taku

AU - Komeda, Yoshibumi

PY - 1997/1/1

Y1 - 1997/1/1

N2 - In rosette plants, the formation of initial flowers is closely linked to the lengthening of internodes (bolting). In order to clarify the molecular basis of bolting, mutants with reduced lengths of internodes were screened. This paper presents the identification and characterization of recessive mutations in ACAULIS5 (ACL5), a gene required for internodal growth in Arabidopsis thaliana. Unlike previously described mutants with reduced size of organs, the acl5 mutant has a severe defect that is restricted to the process of cell elongation after transition to the reproductive stage and shows no phenotype before floral induction. The results of RNA blot hybridizations showed that the acl5 mutation causes a striking reduction in the transcript levels of genes encoding the tonoplast intrinsic protein (γ-TIP) and the endoxyloglucan transferase (EXGT-A1), both of which have recently been suggested to be important for cell elongation. Furthermore, our morphological study indicates that the mutation also causes proliferative arrest of the apical inflorescence meristem. These results strongly suggest that, during the reproductive phase, the wild-type ACL5 gene product has a critical function not only in the control of elongation growth of organs but also in the continued maintenance of the proliferative activity of flower-producing meristems.

AB - In rosette plants, the formation of initial flowers is closely linked to the lengthening of internodes (bolting). In order to clarify the molecular basis of bolting, mutants with reduced lengths of internodes were screened. This paper presents the identification and characterization of recessive mutations in ACAULIS5 (ACL5), a gene required for internodal growth in Arabidopsis thaliana. Unlike previously described mutants with reduced size of organs, the acl5 mutant has a severe defect that is restricted to the process of cell elongation after transition to the reproductive stage and shows no phenotype before floral induction. The results of RNA blot hybridizations showed that the acl5 mutation causes a striking reduction in the transcript levels of genes encoding the tonoplast intrinsic protein (γ-TIP) and the endoxyloglucan transferase (EXGT-A1), both of which have recently been suggested to be important for cell elongation. Furthermore, our morphological study indicates that the mutation also causes proliferative arrest of the apical inflorescence meristem. These results strongly suggest that, during the reproductive phase, the wild-type ACL5 gene product has a critical function not only in the control of elongation growth of organs but also in the continued maintenance of the proliferative activity of flower-producing meristems.

UR - http://www.scopus.com/inward/record.url?scp=0031260033&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031260033&partnerID=8YFLogxK

U2 - 10.1046/j.1365-313X.1997.12040863.x

DO - 10.1046/j.1365-313X.1997.12040863.x

M3 - Article

C2 - 9375398

AN - SCOPUS:0031260033

VL - 12

SP - 863

EP - 874

JO - Plant Journal

JF - Plant Journal

SN - 0960-7412

IS - 4

ER -