TY - GEN
T1 - Achieving uniformity and reproducibility for photonic crystal fluorescence enhanced disease diagnostic microarrays
AU - Race, Caitlin M.
AU - Kwon, Lydia
AU - Cunningham, Brian T.
PY - 2017/1/5
Y1 - 2017/1/5
N2 - Photonic crystal enhanced fluorescence (PCEF) enables a nanostructured dielectric surface to amplify the output of a variety of fluorophore-labeled biomolecule assays for applications that include allergy testing, cancer diagnosis, and viral screening. By increasing fluorescent signals, PCEF enables the achievement of reduced limits of detection with portable and low-cost instrumentation. Expanding the utility of PCEF for disease diagnostics requires the automation, uniformity, and reproducibility of all aspects of the measurement and quantification process, including laser scanning of the PC surface and the subsequent data analysis. In this work, we demonstrate a laser scanning detection instrument, optimal selection of the optical 'on-resonance' incident angle, and automated fluorescence spot intensity analysis that enables PCEF to be utilized for quantification of the concentration of a diagnostic serum antibody (anti-E7) to human papilloma virus infection. The assay is conducted with a single droplet of serum introduced within a PC-integrated microfluidic cartridge that is inserted into the detection instrument.
AB - Photonic crystal enhanced fluorescence (PCEF) enables a nanostructured dielectric surface to amplify the output of a variety of fluorophore-labeled biomolecule assays for applications that include allergy testing, cancer diagnosis, and viral screening. By increasing fluorescent signals, PCEF enables the achievement of reduced limits of detection with portable and low-cost instrumentation. Expanding the utility of PCEF for disease diagnostics requires the automation, uniformity, and reproducibility of all aspects of the measurement and quantification process, including laser scanning of the PC surface and the subsequent data analysis. In this work, we demonstrate a laser scanning detection instrument, optimal selection of the optical 'on-resonance' incident angle, and automated fluorescence spot intensity analysis that enables PCEF to be utilized for quantification of the concentration of a diagnostic serum antibody (anti-E7) to human papilloma virus infection. The assay is conducted with a single droplet of serum introduced within a PC-integrated microfluidic cartridge that is inserted into the detection instrument.
KW - Photonic crystal
KW - disease diagnostics
KW - image processing
KW - microarray screening
UR - http://www.scopus.com/inward/record.url?scp=85011004211&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85011004211&partnerID=8YFLogxK
U2 - 10.1109/ICSENS.2016.7808961
DO - 10.1109/ICSENS.2016.7808961
M3 - Conference contribution
AN - SCOPUS:85011004211
T3 - Proceedings of IEEE Sensors
BT - IEEE Sensors, SENSORS 2016 - Proceedings
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 15th IEEE Sensors Conference, SENSORS 2016
Y2 - 30 October 2016 through 2 November 2016
ER -