Accumulation of the Large and Small Subunits of Ribulose-1,5-Bisphosphate-Carboxylase/Oxygenase during Greening in Euglena gracilis as Determined by Radioimmunoassay

Richard L. Eichholz, Dennis E. Buetow

Research output: Contribution to journalArticle

Abstract

Electrophoretically-separated and purified large and small (L and S) subunits of ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) from Euglena gracilis strain Z were used to generate polyclonal antibodies in rabbits. The monospecific antibodies (immunoglobulin G fractions from blood sera) were used in two different radioimmunoassays to show for the first time the patterns of accumulation of the two rubisco subunits during the greening of dark-grown Euglena exposed to white light. The subunits were detected in dark-grown cells. Following light-exposure, the nuclear-coded cytoplasmically-synthesized S subunit started to accumulate immediately whereas the chloroplastic-encoded and -synthesized L subunit began to accumulate only after 3 to 8 h. After its initial accumulation, S then remained at a constant concentration, while L continued to accumulate. During late greening, both subunits further accumulated. The differential accumulation of the two subunits, especially during early greening, correlates with the finding of a differential accumulation of cytoplasmic versus chloroplastic polysomes following light-stimulation of dark-grown Euglena (Avadhani and Freyssinet, 1989)

Original languageEnglish (US)
Pages (from-to)421-428
Number of pages8
JournalJournal of Plant Physiology
Volume140
Issue number4
DOIs
StatePublished - Jan 1 1992

Keywords

  • Ab-L
  • Ab-S
  • BES
  • CNBr
  • DEAE
  • Euglena
  • G-Ab[R-IgG]
  • HEPES
  • L
  • N,N-his[2-hydroxyethyl]-2-aminoethane sulfonic acid
  • N-2-[hydroxyethyl]-piperazine-N'-[2-ethanesulfonic acid]
  • R-IgG
  • R-IgG against L
  • R-IgG against S
  • Ribulose-1,5-bisphosphate-carboxylase/oxygenase
  • S
  • cyanogen bromide (activated)
  • diethylaminoethyl;DTT;dithiothreitol
  • goat antirabbit IgG
  • large subunit of rubisco
  • light-induced greening
  • rabbit immunoglobulin G
  • radioimmunoassay
  • regulation of subunit synthesis
  • ribulose-1,5-bisphosphate-carboxylase/oxygenase
  • rubisco
  • small subunit of rubisco

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

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