Abstract
Throughout the biological sciences, the past 15 years have seen a push toward the analysis and engineering of biological systems at the organism level. Given the complexity of even the simplest organisms, though, to elicit a phenotype of interest often requires genotypic manipulation of several loci. By traditional means, sequential editing of genomic targets requires a significant investment of time and labor, as the desired editing event typically occurs at a very low frequency against an overwhelming unedited background. In recent years, the development of a suite of new techniques has greatly increased editing efficiency, opening up the possibility for multiple editing events to occur in parallel. Termed as multiplexed genome engineering, this approach to genome editing has greatly expanded the scope of possible genome manipulations in diverse hosts, ranging from bacteria to human cells. The enabling technologies for multiplexed genome engineering include oligonucleotide-based and nuclease-based methodologies, and their application has led to the great breadth of successful examples described in this review. While many technical challenges remain, there also exists a multiplicity of opportunities in this rapidly expanding field. WIREs Syst Biol Med 2016, 8:5-21. doi: 10.1002/wsbm.1319 For further resources related to this article, please visit the WIREs website.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 5-21 |
| Number of pages | 17 |
| Journal | Wiley Interdisciplinary Reviews: Systems Biology and Medicine |
| Volume | 8 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 2016 |
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ASJC Scopus subject areas
- Medicine (miscellaneous)
- Biochemistry, Genetics and Molecular Biology (miscellaneous)
Cite this
Accelerated genome engineering through multiplexing. / Bao, Zehua; Cobb, Ryan E.; Zhao, Huimin.
In: Wiley Interdisciplinary Reviews: Systems Biology and Medicine, Vol. 8, No. 1, 01.01.2016, p. 5-21.Research output: Contribution to journal › Review article
}
TY - JOUR
T1 - Accelerated genome engineering through multiplexing
AU - Bao, Zehua
AU - Cobb, Ryan E.
AU - Zhao, Huimin
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Throughout the biological sciences, the past 15 years have seen a push toward the analysis and engineering of biological systems at the organism level. Given the complexity of even the simplest organisms, though, to elicit a phenotype of interest often requires genotypic manipulation of several loci. By traditional means, sequential editing of genomic targets requires a significant investment of time and labor, as the desired editing event typically occurs at a very low frequency against an overwhelming unedited background. In recent years, the development of a suite of new techniques has greatly increased editing efficiency, opening up the possibility for multiple editing events to occur in parallel. Termed as multiplexed genome engineering, this approach to genome editing has greatly expanded the scope of possible genome manipulations in diverse hosts, ranging from bacteria to human cells. The enabling technologies for multiplexed genome engineering include oligonucleotide-based and nuclease-based methodologies, and their application has led to the great breadth of successful examples described in this review. While many technical challenges remain, there also exists a multiplicity of opportunities in this rapidly expanding field. WIREs Syst Biol Med 2016, 8:5-21. doi: 10.1002/wsbm.1319 For further resources related to this article, please visit the WIREs website.
AB - Throughout the biological sciences, the past 15 years have seen a push toward the analysis and engineering of biological systems at the organism level. Given the complexity of even the simplest organisms, though, to elicit a phenotype of interest often requires genotypic manipulation of several loci. By traditional means, sequential editing of genomic targets requires a significant investment of time and labor, as the desired editing event typically occurs at a very low frequency against an overwhelming unedited background. In recent years, the development of a suite of new techniques has greatly increased editing efficiency, opening up the possibility for multiple editing events to occur in parallel. Termed as multiplexed genome engineering, this approach to genome editing has greatly expanded the scope of possible genome manipulations in diverse hosts, ranging from bacteria to human cells. The enabling technologies for multiplexed genome engineering include oligonucleotide-based and nuclease-based methodologies, and their application has led to the great breadth of successful examples described in this review. While many technical challenges remain, there also exists a multiplicity of opportunities in this rapidly expanding field. WIREs Syst Biol Med 2016, 8:5-21. doi: 10.1002/wsbm.1319 For further resources related to this article, please visit the WIREs website.
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U2 - 10.1002/wsbm.1319
DO - 10.1002/wsbm.1319
M3 - Review article
C2 - 26394307
AN - SCOPUS:84955216261
VL - 8
SP - 5
EP - 21
JO - Wiley Interdisciplinary Reviews: Systems Biology and Medicine
JF - Wiley Interdisciplinary Reviews: Systems Biology and Medicine
SN - 1939-5094
IS - 1
ER -