Accelerated diffusion methods for inorganic-nitrogen analysis of soil extracts and water

S. A. Khan, R. L. Mulvaney, C. S. Mulvaney

Research output: Contribution to journalArticlepeer-review

Abstract

The major limitation of diffusion methods for N analysis is the lime required to obtain the results - typically from one to several days. Methods were developed that involve a much shorter period fur diffusion. In these methods, 10- to 100-mL samples of 2 or 4 M KCl soil extract or water were treated with MgO, or MgO plus Devarda's alloy, in a 473-mL (1-pint) wide-mouth Mason jar to liberate NH3-N4 NO3-N4 and/or NO2-N as NH3. The jar was placed on an electric griddle maintained at 45 to 50°C. Gaseous NH3 was collected in 5 mL of H3BO3 indicator solution in a petri dish attached to the Mason jar lid, for quantitative determination by acidimetric titration. Complete recovery of 4 mg of N was achieved from 10 or 20 mL in 1.5 to 5 h. Recovery from 50 or 100 mL was complete in 5 to 8.5 h with 1 to 2 mg of N. The methods permit 13N analysis of the diffused NH3-N. Isotopic analyses of labeled soil extracts were accurate to within 3%, as determined by isotope-dilution calculations. Diffusions from 10 to 50 mL were subject to very little, if any, interference from decomposition of alkali-labile organic-N compounds.

Original languageEnglish (US)
Pages (from-to)936-942
Number of pages7
JournalSoil Science Society of America Journal
Volume61
Issue number3
DOIs
StatePublished - Jan 1 1997

ASJC Scopus subject areas

  • Soil Science

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