Aberrant gene expression patterns in placentomes are associated with phenotypically normal and abnormal cattle cloned by somatic cell nuclear transfer

Robin E. Everts, Pascale Chavatte-Palmer, Anthony Razzak, Isabelle Hue, Cheryl A. Green, Rosane Oliveira, Xavier Vignon, Sandra L. Rodriguez-Zas, X. Cindy Tian, Xiangzhong Yang, Jean Paul Renard, Harris A. Lewin

Research output: Contribution to journalArticle

Abstract

Transcription profiling of placentomes derived from somatic cell nuclear transfer (SCNT, n = 20), in vitro fertilization (IVF, n = 9), and artificial insemination (AI, n = 9) at or near term development was performed to better understand why SCNT and IVF often result in placental defects, hydrops, and large offspring syndrome (LOS). Multivariate analysis of variance was used to distinguish the effects of SCNT, IVF, and AI on gene expression, taking into account the effects of parturition (term or preterm), sex of fetus, breed of dam, breed of fetus, and pathological finding in the offspring (hydrops, normal, or other abnormalities). Differential expression of 20 physiologically important genes was confirmed with quantitative PCR. The largest effect on placentome gene expression was attributable to whether placentas were collected at term or preterm (i.e., whether the collection was because of disease or to obtain stage-matched controls) followed by placentome source (AI, IVF, or SCNT). Gene expression in SCNT placentomes was dramatically different from AI (n = 336 genes; 276 >2-fold) and from IVF (n = 733 genes; 162 >2-fold) placentomes. Functional analysis of differentially expressed genes (DEG) showed that IVF has significant effects on genes associated with cellular metabolism. In contrast, DEG associated with SCNT are involved in multiple pathways, including cell cycle, cell death, and gene expression. Many DEG were shared between the gene lists for IVF and SCNT comparisons, suggesting that common pathways are affected by the embryo culture methods used for IVF and SCNT. However, the many unique gene functions and pathways affected by SCNT suggest that cloned fetuses may be starved and accumulating toxic wastes due to placental insufficiency caused by reprogramming errors. Many of these genes are candidates for hydrops and LOS.

Original languageEnglish (US)
Pages (from-to)65-77
Number of pages13
JournalPhysiological genomics
Volume33
Issue number1
DOIs
StatePublished - Mar 14 2008

Fingerprint

Placenta
Gene Expression
Genes
Edema
Fetus
Placental Insufficiency
cdc Genes
Artificial Insemination
Poisons
Fertilization in Vitro
Analysis of Variance
Cell Death
Multivariate Analysis
Embryonic Structures
Parturition
Polymerase Chain Reaction

Keywords

  • Bovine
  • Placenta
  • Reprogramming

ASJC Scopus subject areas

  • Physiology
  • Genetics

Cite this

Aberrant gene expression patterns in placentomes are associated with phenotypically normal and abnormal cattle cloned by somatic cell nuclear transfer. / Everts, Robin E.; Chavatte-Palmer, Pascale; Razzak, Anthony; Hue, Isabelle; Green, Cheryl A.; Oliveira, Rosane; Vignon, Xavier; Rodriguez-Zas, Sandra L.; Tian, X. Cindy; Yang, Xiangzhong; Renard, Jean Paul; Lewin, Harris A.

In: Physiological genomics, Vol. 33, No. 1, 14.03.2008, p. 65-77.

Research output: Contribution to journalArticle

Everts, RE, Chavatte-Palmer, P, Razzak, A, Hue, I, Green, CA, Oliveira, R, Vignon, X, Rodriguez-Zas, SL, Tian, XC, Yang, X, Renard, JP & Lewin, HA 2008, 'Aberrant gene expression patterns in placentomes are associated with phenotypically normal and abnormal cattle cloned by somatic cell nuclear transfer', Physiological genomics, vol. 33, no. 1, pp. 65-77. https://doi.org/10.1152/physiolgenomics.00223.2007
Everts, Robin E. ; Chavatte-Palmer, Pascale ; Razzak, Anthony ; Hue, Isabelle ; Green, Cheryl A. ; Oliveira, Rosane ; Vignon, Xavier ; Rodriguez-Zas, Sandra L. ; Tian, X. Cindy ; Yang, Xiangzhong ; Renard, Jean Paul ; Lewin, Harris A. / Aberrant gene expression patterns in placentomes are associated with phenotypically normal and abnormal cattle cloned by somatic cell nuclear transfer. In: Physiological genomics. 2008 ; Vol. 33, No. 1. pp. 65-77.
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