Abstract
The inherent architectural and chemical complexities of microbial biofilms mask our understanding of how these communities form, survive, propagate, and influence their surrounding environment. Here we describe a simple and versatile workflow for the cultivation and characterization of model flow-cell-based microbial ecosystems. A customized low-shear drip flow reactor was designed and employed to cultivate single and coculture flow-cell biofilms at the air-liquid interface of several metal surfaces. Pseudomonas putida F1 and Shewanella oneidensis MR-1 were selected as model organisms for this study. The utility and versatility of this platform was demonstrated via the application of several chemical and morphological imaging techniques - including matrix-assisted laser desorption/ionization mass spectrometry imaging, secondary ion mass spectrometry imaging, and scanning electron microscopy - and through the examination of model systems grown on iron substrates of varying compositions. Implementation of these techniques in combination with tandem mass spectrometry and a two-step imaging principal component analysis strategy resulted in the identification and characterization of 23 lipids and 3 oligosaccharides in P. putida F1 biofilms, the discovery of interaction-specific analytes, and the observation of several variations in cell and substrate morphology present during microbially influenced corrosion. The presented workflow is well-suited for examination of both single and multispecies drip flow biofilms and offers a platform for fundamental inquiries into biofilm formation, microbe-microbe interactions, and microbially influenced corrosion.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 6725-6734 |
| Number of pages | 10 |
| Journal | Analytical chemistry |
| Volume | 90 |
| Issue number | 11 |
| DOIs | |
| State | Published - Jun 5 2018 |
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ASJC Scopus subject areas
- Analytical Chemistry
Cite this
A Versatile Strategy for Characterization and Imaging of Drip Flow Microbial Biofilms. / Li, Bin; Dunham, Sage J.B.; Ellis, Joseph F.; Lange, Justin D.; Smith, Justin R.; Yang, Ning; King, Travis L.; Amaya, Kensey R.; Arnett, Clint M.; Sweedler, Jonathan V.
In: Analytical chemistry, Vol. 90, No. 11, 05.06.2018, p. 6725-6734.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A Versatile Strategy for Characterization and Imaging of Drip Flow Microbial Biofilms
AU - Li, Bin
AU - Dunham, Sage J.B.
AU - Ellis, Joseph F.
AU - Lange, Justin D.
AU - Smith, Justin R.
AU - Yang, Ning
AU - King, Travis L.
AU - Amaya, Kensey R.
AU - Arnett, Clint M.
AU - Sweedler, Jonathan V.
PY - 2018/6/5
Y1 - 2018/6/5
N2 - The inherent architectural and chemical complexities of microbial biofilms mask our understanding of how these communities form, survive, propagate, and influence their surrounding environment. Here we describe a simple and versatile workflow for the cultivation and characterization of model flow-cell-based microbial ecosystems. A customized low-shear drip flow reactor was designed and employed to cultivate single and coculture flow-cell biofilms at the air-liquid interface of several metal surfaces. Pseudomonas putida F1 and Shewanella oneidensis MR-1 were selected as model organisms for this study. The utility and versatility of this platform was demonstrated via the application of several chemical and morphological imaging techniques - including matrix-assisted laser desorption/ionization mass spectrometry imaging, secondary ion mass spectrometry imaging, and scanning electron microscopy - and through the examination of model systems grown on iron substrates of varying compositions. Implementation of these techniques in combination with tandem mass spectrometry and a two-step imaging principal component analysis strategy resulted in the identification and characterization of 23 lipids and 3 oligosaccharides in P. putida F1 biofilms, the discovery of interaction-specific analytes, and the observation of several variations in cell and substrate morphology present during microbially influenced corrosion. The presented workflow is well-suited for examination of both single and multispecies drip flow biofilms and offers a platform for fundamental inquiries into biofilm formation, microbe-microbe interactions, and microbially influenced corrosion.
AB - The inherent architectural and chemical complexities of microbial biofilms mask our understanding of how these communities form, survive, propagate, and influence their surrounding environment. Here we describe a simple and versatile workflow for the cultivation and characterization of model flow-cell-based microbial ecosystems. A customized low-shear drip flow reactor was designed and employed to cultivate single and coculture flow-cell biofilms at the air-liquid interface of several metal surfaces. Pseudomonas putida F1 and Shewanella oneidensis MR-1 were selected as model organisms for this study. The utility and versatility of this platform was demonstrated via the application of several chemical and morphological imaging techniques - including matrix-assisted laser desorption/ionization mass spectrometry imaging, secondary ion mass spectrometry imaging, and scanning electron microscopy - and through the examination of model systems grown on iron substrates of varying compositions. Implementation of these techniques in combination with tandem mass spectrometry and a two-step imaging principal component analysis strategy resulted in the identification and characterization of 23 lipids and 3 oligosaccharides in P. putida F1 biofilms, the discovery of interaction-specific analytes, and the observation of several variations in cell and substrate morphology present during microbially influenced corrosion. The presented workflow is well-suited for examination of both single and multispecies drip flow biofilms and offers a platform for fundamental inquiries into biofilm formation, microbe-microbe interactions, and microbially influenced corrosion.
UR - http://www.scopus.com/inward/record.url?scp=85046676705&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85046676705&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.8b00560
DO - 10.1021/acs.analchem.8b00560
M3 - Article
C2 - 29723465
AN - SCOPUS:85046676705
VL - 90
SP - 6725
EP - 6734
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 11
ER -