A vacuum-vortex technique for preparation of anoxic solutions or liquid culture media in small volumes for cultivating methanogens or other strict anaerobes

Ralph S. Wolfe; William W. Metcalf

doi:10.1016/j.anaerobe.2009.11.005

A vacuum-vortex technique for preparation of anoxic solutions or liquid culture media in small volumes for cultivating methanogens or other strict anaerobes

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Abstract

A highly efficient method is described for producing at room temperature anoxic solutions of 50 ml or less in test tubes or serum vials by combining negative pressure with strong vortexing so that the liquid-surface, gas exchange area is increased by orders of magnitude. Liquid media suitable for the cultivation of methanogens may be rendered anoxic after three short vacuum-vortex steps.

Original language	English (US)
Pages (from-to)	216-219
Number of pages	4
Journal	Anaerobe
Volume	16
Issue number	3
DOIs	https://doi.org/10.1016/j.anaerobe.2009.11.005
State	Published - Jun 2010

Keywords

Anoxic media
Anoxic solutions
Methanogens
Vacuum-vortex technique

ASJC Scopus subject areas

Microbiology
Infectious Diseases

Online availability

10.1016/j.anaerobe.2009.11.005

Library availability

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AU - Metcalf, William W.

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KW - Anoxic solutions

KW - Methanogens

KW - Vacuum-vortex technique

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A vacuum-vortex technique for preparation of anoxic solutions or liquid culture media in small volumes for cultivating methanogens or other strict anaerobes

Abstract

Keywords

ASJC Scopus subject areas

Online availability

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