TY - JOUR
T1 - A ubiquitous Plasmodium protein displays a unique surface labeling pattern in sporozoites
AU - LaCrue, Alexis N.
AU - Sivaguru, Mayandi
AU - Walter, Marika F.
AU - Fidock, David A.
AU - James, Anthony A.
AU - Beerntsen, Brenda T.
N1 - Funding Information:
The partial P. reichenowi sequence data were produced by the Pathogen Sequencing Unit at the Wellcome Trust Sanger Institute and can be obtained from http://www.sanger.ac.uk/Projects/P_reichenowi/ . This study was supported by the National Institutes of Health (NIH) grants AI 01657, AI 53156, and F32 AI 09731 to B.T.B and a Minority Biomedical Research Support (MBRTI)/NIH grant (R25 GM56901) and Missouri Alliance for Graduate Education and the Professoriate (MAGEP)/NSF Fellowship to A.N.L.
PY - 2006/8
Y1 - 2006/8
N2 - The Plasmodium sporozoite is infective for mosquito salivary glands and vertebrate host tissues. Although it is a key developmental stage of the malaria parasite, relatively few sporozoite surface or secreted proteins have been identified and characterized. Herein, we describe the molecular and cellular characterization of a novel surface molecule that is preferentially-expressed in salivary gland sporozoites as compared to oocyst and hemolymph sporozoites. This molecule, designated the sporozoite and erythrocytic stages (SES) protein (formerly known as Pg4), exhibits a spiral surface labeling pattern that spans over a known sporozoite surface antigen, the circumsporozoite protein, with only minor co-localization. SES consists of 551 amino acids encoding a putative 63.2 kDa protein that has been shown to be expressed not only on particular sporozoite stages, but also during the asexual and gametocyte stages. This novel protein also has three domains of unknown function that are conserved in at least eight Plasmodium spp. that represent human, avian, non-human primate, and rodent malarias.
AB - The Plasmodium sporozoite is infective for mosquito salivary glands and vertebrate host tissues. Although it is a key developmental stage of the malaria parasite, relatively few sporozoite surface or secreted proteins have been identified and characterized. Herein, we describe the molecular and cellular characterization of a novel surface molecule that is preferentially-expressed in salivary gland sporozoites as compared to oocyst and hemolymph sporozoites. This molecule, designated the sporozoite and erythrocytic stages (SES) protein (formerly known as Pg4), exhibits a spiral surface labeling pattern that spans over a known sporozoite surface antigen, the circumsporozoite protein, with only minor co-localization. SES consists of 551 amino acids encoding a putative 63.2 kDa protein that has been shown to be expressed not only on particular sporozoite stages, but also during the asexual and gametocyte stages. This novel protein also has three domains of unknown function that are conserved in at least eight Plasmodium spp. that represent human, avian, non-human primate, and rodent malarias.
KW - Malaria
KW - Preferential expression
KW - Sporozoite
KW - Surface protein
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U2 - 10.1016/j.molbiopara.2006.03.016
DO - 10.1016/j.molbiopara.2006.03.016
M3 - Article
C2 - 16697061
AN - SCOPUS:33745353803
VL - 148
SP - 199
EP - 209
JO - Molecular and Biochemical Parasitology
JF - Molecular and Biochemical Parasitology
SN - 0166-6851
IS - 2
ER -