A turnip mosaic virus determinant of systemic necrosis in nicotiana benthamiana and a novel resistance-breaking determinant in Chinese cabbage identified from chimeric infectious clones

Ik Hyun Kim, Hye Kyoung Ju, Junsu Gong, Jae Yeong Han, Eun Young Seo, Sang Won Cho, Wen Xing Hu, Su Ryun Choi, Yong Pyo Lim, Leslie L Domier, John Hammond, Hyoun Sub Lim

Research output: Contribution to journalArticle

Abstract

Infectious clones of Korean turnip mosaic virus (TuMV) isolates KIH1 and HJY1 share 88.1% genomic nucleotides and 96.4% polyprotein amino acid identity, and they induce systemic necrosis or mild mosaic, respectively, in Nicotiana benthamiana. Chimeric constructs between these isolates exchanged the 59, central, and 39 domains of KIH1 (K) and HJY1 (H), where the order of the letters indicates the origin of these domains. KIH1 and chimeras KHH and KKH induced systemic necrosis, whereas HJY1 and chimeras HHK, HKK, and HKH induced mild symptoms, indicating the determinant of necrosis to be within the 59 3.9 kb of KIH1; amino acid identities of the included P1, Helper component protease, P3, 6K1, and cylindrical inclusion N-terminal domain were 90.06, 98.91, 93.80, 100, and 100%, respectively. Expression of P1 or P3 from a potato virus X vector yielded symptom differences only between P3 of KIH1 and HJY1, implicating a role for P3 in necrosis in N. benthamiana. Chimera KKH infected Brassica rapa var. pekinensis 'Norang', which was resistant to both KIH1 and HJY1, indicating that two separate TuMV determinants are required to overcome the resistance. Ability of diverse TuMV isolates, chimeras, and recombinants to overcome resistance in breeding lines may allow identification of novel resistance genes.

Original languageEnglish (US)
Pages (from-to)1638-1647
Number of pages10
JournalPhytopathology
Volume109
Issue number9
DOIs
StatePublished - Jan 1 2019

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Turnip mosaic virus
Chinese cabbage
Nicotiana benthamiana
chimerism
necrosis
clones
Potato virus X
amino acids
Brassica rapa
breeding lines
proteinases
nucleotides
genomics
genes

Keywords

  • Full-length cdna infectious clones
  • Pathogenicity
  • Phylogenetic analysis

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

A turnip mosaic virus determinant of systemic necrosis in nicotiana benthamiana and a novel resistance-breaking determinant in Chinese cabbage identified from chimeric infectious clones. / Kim, Ik Hyun; Ju, Hye Kyoung; Gong, Junsu; Han, Jae Yeong; Seo, Eun Young; Cho, Sang Won; Hu, Wen Xing; Choi, Su Ryun; Lim, Yong Pyo; Domier, Leslie L; Hammond, John; Lim, Hyoun Sub.

In: Phytopathology, Vol. 109, No. 9, 01.01.2019, p. 1638-1647.

Research output: Contribution to journalArticle

Kim, Ik Hyun ; Ju, Hye Kyoung ; Gong, Junsu ; Han, Jae Yeong ; Seo, Eun Young ; Cho, Sang Won ; Hu, Wen Xing ; Choi, Su Ryun ; Lim, Yong Pyo ; Domier, Leslie L ; Hammond, John ; Lim, Hyoun Sub. / A turnip mosaic virus determinant of systemic necrosis in nicotiana benthamiana and a novel resistance-breaking determinant in Chinese cabbage identified from chimeric infectious clones. In: Phytopathology. 2019 ; Vol. 109, No. 9. pp. 1638-1647.
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abstract = "Infectious clones of Korean turnip mosaic virus (TuMV) isolates KIH1 and HJY1 share 88.1{\%} genomic nucleotides and 96.4{\%} polyprotein amino acid identity, and they induce systemic necrosis or mild mosaic, respectively, in Nicotiana benthamiana. Chimeric constructs between these isolates exchanged the 59, central, and 39 domains of KIH1 (K) and HJY1 (H), where the order of the letters indicates the origin of these domains. KIH1 and chimeras KHH and KKH induced systemic necrosis, whereas HJY1 and chimeras HHK, HKK, and HKH induced mild symptoms, indicating the determinant of necrosis to be within the 59 3.9 kb of KIH1; amino acid identities of the included P1, Helper component protease, P3, 6K1, and cylindrical inclusion N-terminal domain were 90.06, 98.91, 93.80, 100, and 100{\%}, respectively. Expression of P1 or P3 from a potato virus X vector yielded symptom differences only between P3 of KIH1 and HJY1, implicating a role for P3 in necrosis in N. benthamiana. Chimera KKH infected Brassica rapa var. pekinensis 'Norang', which was resistant to both KIH1 and HJY1, indicating that two separate TuMV determinants are required to overcome the resistance. Ability of diverse TuMV isolates, chimeras, and recombinants to overcome resistance in breeding lines may allow identification of novel resistance genes.",
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TY - JOUR

T1 - A turnip mosaic virus determinant of systemic necrosis in nicotiana benthamiana and a novel resistance-breaking determinant in Chinese cabbage identified from chimeric infectious clones

AU - Kim, Ik Hyun

AU - Ju, Hye Kyoung

AU - Gong, Junsu

AU - Han, Jae Yeong

AU - Seo, Eun Young

AU - Cho, Sang Won

AU - Hu, Wen Xing

AU - Choi, Su Ryun

AU - Lim, Yong Pyo

AU - Domier, Leslie L

AU - Hammond, John

AU - Lim, Hyoun Sub

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Infectious clones of Korean turnip mosaic virus (TuMV) isolates KIH1 and HJY1 share 88.1% genomic nucleotides and 96.4% polyprotein amino acid identity, and they induce systemic necrosis or mild mosaic, respectively, in Nicotiana benthamiana. Chimeric constructs between these isolates exchanged the 59, central, and 39 domains of KIH1 (K) and HJY1 (H), where the order of the letters indicates the origin of these domains. KIH1 and chimeras KHH and KKH induced systemic necrosis, whereas HJY1 and chimeras HHK, HKK, and HKH induced mild symptoms, indicating the determinant of necrosis to be within the 59 3.9 kb of KIH1; amino acid identities of the included P1, Helper component protease, P3, 6K1, and cylindrical inclusion N-terminal domain were 90.06, 98.91, 93.80, 100, and 100%, respectively. Expression of P1 or P3 from a potato virus X vector yielded symptom differences only between P3 of KIH1 and HJY1, implicating a role for P3 in necrosis in N. benthamiana. Chimera KKH infected Brassica rapa var. pekinensis 'Norang', which was resistant to both KIH1 and HJY1, indicating that two separate TuMV determinants are required to overcome the resistance. Ability of diverse TuMV isolates, chimeras, and recombinants to overcome resistance in breeding lines may allow identification of novel resistance genes.

AB - Infectious clones of Korean turnip mosaic virus (TuMV) isolates KIH1 and HJY1 share 88.1% genomic nucleotides and 96.4% polyprotein amino acid identity, and they induce systemic necrosis or mild mosaic, respectively, in Nicotiana benthamiana. Chimeric constructs between these isolates exchanged the 59, central, and 39 domains of KIH1 (K) and HJY1 (H), where the order of the letters indicates the origin of these domains. KIH1 and chimeras KHH and KKH induced systemic necrosis, whereas HJY1 and chimeras HHK, HKK, and HKH induced mild symptoms, indicating the determinant of necrosis to be within the 59 3.9 kb of KIH1; amino acid identities of the included P1, Helper component protease, P3, 6K1, and cylindrical inclusion N-terminal domain were 90.06, 98.91, 93.80, 100, and 100%, respectively. Expression of P1 or P3 from a potato virus X vector yielded symptom differences only between P3 of KIH1 and HJY1, implicating a role for P3 in necrosis in N. benthamiana. Chimera KKH infected Brassica rapa var. pekinensis 'Norang', which was resistant to both KIH1 and HJY1, indicating that two separate TuMV determinants are required to overcome the resistance. Ability of diverse TuMV isolates, chimeras, and recombinants to overcome resistance in breeding lines may allow identification of novel resistance genes.

KW - Full-length cdna infectious clones

KW - Pathogenicity

KW - Phylogenetic analysis

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U2 - 10.1094/PHYTO-08-18-0323-R

DO - 10.1094/PHYTO-08-18-0323-R

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C2 - 31044662

AN - SCOPUS:85071457034

VL - 109

SP - 1638

EP - 1647

JO - Phytopathology

JF - Phytopathology

SN - 0031-949X

IS - 9

ER -