A tRNA(Glu) gene from the hyperthermophilic archaeon Pyrococcus furiosus contains the 3'-terminal CCA sequence of the mature tRNA

Isaac Cann, Yoshizumi Ishino

Research output: Contribution to journalArticlepeer-review

Abstract

We cloned a gene encoding tRNA(Glu) of the hyperthermophilic archaeon Pyrococcus furiosus. This gene contains the CCA sequence corresponding to the 3'-terminus of the mature tRNA. It is known that, like in eukaryal tRNAs, the CCA-termini of' archaeal tRNAs are generally not encoded. Therefore, we analyzed all tRNA genes in the genome of Methanococcus jannaschii estimated by its whole genome sequence. Twenty-one of 37 listed tRNA genes contained the 3'-terminal CCA sequence. The corresponding M. jannaschii tRNA(Glu) gene does not contain the CCA sequence, although the tRNA sequences or the M. jannaschii and P. furiosus tRNA(Glu) genes are 86% identical.

Original languageEnglish (US)
Pages (from-to)199-204
Number of pages6
JournalFEMS Microbiology Letters
Volume160
Issue number2
DOIs
StatePublished - Mar 15 1998
Externally publishedYes

Keywords

  • Archaea
  • CCA-adding enzyme
  • Gene organization
  • Methanococcus jannaschii

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

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