A Triticum tauschii protein kinase related to wheat PKABA1 is associated with ABA signaling and is distributed between the nucleus and cytosol

Lynn D. Holappa, M. K. Walker-Simmons, T. H.D. Ho, Dean Edward Riechers, Diane M. Beckles, Russell L. Jones

Research output: Contribution to journalArticle

Abstract

A new member of the cereal PKABA1 subfamily of protein kinases, TtPK1, was isolated from Triticum tauschii, a diploid progenitor of hexaploid wheat, Triticum aestivum. The full-length TtPK1 cDNA was cloned from a library derived from vegetative tissues from 26 d old light grown T. tauschii seedlings. TtPK1 cDNA hybridizes to transcripts that are upregulated in dehydrated leaves and are abundant in coleoptile tissue of 7 d old T. aestivum seedlings. TtPK1 mRNA has nucleotide identities of 82 and 79% to PKABA1 and TaPK3, respectively, and deduced amino acid sequence identities of 84 and 83% to PKABA1 and TaPK3, respectively. TtPK1 is similar to members of the SnRK2 subfamily of protein kinases in that it contains a unique acidic domain at the carboxyl terminus, and all twelve of the conserved subdomains found in serine/threonine protein kinases. Functional analyses of TtPK1 transiently over-expressed by bombarding barley half-grains showed that TtPK1 could suppress gibberellic acid inducible alpha-amylase gene expression, a suppressive activity similar to that of both PKABA1 and ABA. When transiently expressed in barley aleurone protoplasts, TtPK1-GFP accumulates in the nucleus and cytosol while a mutant TtPK1-GFP was localized only to the cytoplasm and vacuoles.

Original languageEnglish (US)
Pages (from-to)333-346
Number of pages14
JournalJournal of Cereal Science
Volume41
Issue number3
DOIs
StatePublished - May 2005

Fingerprint

Triticum
cytosol
protein kinases
Cytosol
Protein Kinases
abscisic acid
Triticum aestivum
Complementary DNA
barley
Tissue
wheat
seedlings
alpha-Amylases
Protein-Serine-Threonine Kinases
coleoptiles
Hordeum
hexaploidy
Seedlings
gibberellic acid
alpha-amylase

Keywords

  • ABA
  • Dehydration
  • GFP
  • PKABA1
  • Protein kinase
  • SNRK
  • Triticum tauschii

ASJC Scopus subject areas

  • Food Science
  • Biochemistry

Cite this

A Triticum tauschii protein kinase related to wheat PKABA1 is associated with ABA signaling and is distributed between the nucleus and cytosol. / Holappa, Lynn D.; Walker-Simmons, M. K.; Ho, T. H.D.; Riechers, Dean Edward; Beckles, Diane M.; Jones, Russell L.

In: Journal of Cereal Science, Vol. 41, No. 3, 05.2005, p. 333-346.

Research output: Contribution to journalArticle

Holappa, Lynn D. ; Walker-Simmons, M. K. ; Ho, T. H.D. ; Riechers, Dean Edward ; Beckles, Diane M. ; Jones, Russell L. / A Triticum tauschii protein kinase related to wheat PKABA1 is associated with ABA signaling and is distributed between the nucleus and cytosol. In: Journal of Cereal Science. 2005 ; Vol. 41, No. 3. pp. 333-346.
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