A transposable partitioning locus used to stabilize plasmidborne hydrogen oxidation and trifolitoxin production genes in a Sinorhizobium strain

Angela D. Kent, Michelle L. Wojtasiak, Eduardo A. Robleto, Eric W. Triplett

Research output: Contribution to journalArticlepeer-review

Abstract

Improved nitrogen-fixing inoculum strains for leguminous crops must be able to effectively compete with indigenous strains for nodulation, enhance legume productivity compared to the productivity obtained with indigenous strains, and maintain stable expression of any added genes in the absence of selection pressure. We constructed a transposable element containing the tfx region for expression of increased nodulation competitiveness and the par locus for plasmid stability. The transposon was inserted into tetA of pHU52, a broadhost-range plasmid conferring the H2 uptake phenotype. The resulting plasmid, pHUTFXPAR, conferred the plasmid stability, trifolitoxin production, and H2 uptake phenotypes in the broad-host-range organism Sinorhizobium sp. strain ANU280. The broad applications of a transposon conferring plasmid stability are discussed.

Original languageEnglish (US)
Pages (from-to)1657-1662
Number of pages6
JournalApplied and environmental microbiology
Volume64
Issue number5
DOIs
StatePublished - May 1998
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Ecology
  • Applied Microbiology and Biotechnology

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