A transcriptomic atlas of acute stress response to low pH in multiple Issatchenkia orientalis strains

Veronika Dubinkina, Shounak Bhogale, Ping Hung Hsieh, Payam Dibaeinia, Ananthan Nambiar, Sergei Maslov, Yasuo Yoshikuni, Saurabh Sinha

Research output: Contribution to journalArticlepeer-review

Abstract

IMPORTANCE: Issatchenkia orientalis is a promising industrial chassis to produce biofuels and bioproducts due to its high tolerance to multiple environmental stresses such as low pH, heat, and other chemicals otherwise toxic for the most widely used microbes. Yet, little is known about specific mechanisms of such tolerance in this organism, hindering our ability to engineer this species to produce valuable biochemicals. Here, we report a comprehensive study of the mechanisms of acidic tolerance in this species via transcriptome profiling across variable pH for 12 different strains with different phenotypes. We found multiple regulatory mechanisms involved in tolerance to low pH in different strains of I. orientalis, marking potential targets for future gene editing and perturbation experiments.

Original languageEnglish (US)
Pages (from-to)e0253623
JournalMicrobiology Spectrum
Volume12
Issue number1
DOIs
StatePublished - Jan 11 2024

Keywords

  • Issatchenkia orientalis
  • acidic stress
  • gene regulation
  • low pH

ASJC Scopus subject areas

  • Physiology
  • Ecology
  • Immunology and Microbiology(all)
  • Genetics
  • Microbiology (medical)
  • Cell Biology
  • Infectious Diseases

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