A traction force threshold signifies metastatic phenotypic change in multicellular epithelia

Yao Zhang, Xuechen Shi, Tiankai Zhao, Changjin Huang, Qiong Wei, Xin Tang, Lorraine C. Santy, M Taher A Saif, Sulin Zhang

Research output: Contribution to journalArticle

Abstract

Cancer metastasis has been believed as a genetically programmed process that is commonly marked by biochemical signals. Here using extracellular matrix control of cellular mechanics, we establish that cellular force threshold can also mark in vitro metastatic phenotypic change and malignant transformation in HCT-8 cell colonies. We observe that for prolonged culture time the HCT-8 cell colonies disperse into individual malignant cells, and the metastatic-like dispersion depends on both cell-seeding gel stiffness and colony size. Cellular force microscopies show that gel stiffness and colony size are also two key parameters that modulate cellular forces, suggesting the correlations between the cellular forces and the metastatic phenotypic change. Using our recently developed biophysical model, we construct an extracellular traction phase diagram in the stiffness-size space, filled with experimental data on the colony behavior. From the phase diagram we identify a phase boundary as a traction force threshold above which the metastatic phenotypic transition occurs and below which the cell colonies remain cohesive. Our finding suggests that the traction threshold can be regarded as an effective mechano-marker for the onset of the metastatic-like dispersion and malignant transformation.

Original languageEnglish (US)
Pages (from-to)7203-7210
Number of pages8
JournalSoft Matter
Volume15
Issue number36
DOIs
StatePublished - Jan 1 2019

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epithelium
traction
Stiffness
Phase diagrams
thresholds
Gels
stiffness
cells
Traction (friction)
Phase boundaries
Microscopic examination
Mechanics
phase diagrams
gels
metastasis
inoculation
markers
cancer
microscopy
matrices

ASJC Scopus subject areas

  • Chemistry(all)
  • Condensed Matter Physics

Cite this

Zhang, Y., Shi, X., Zhao, T., Huang, C., Wei, Q., Tang, X., ... Zhang, S. (2019). A traction force threshold signifies metastatic phenotypic change in multicellular epithelia. Soft Matter, 15(36), 7203-7210. https://doi.org/10.1039/c9sm00733d

A traction force threshold signifies metastatic phenotypic change in multicellular epithelia. / Zhang, Yao; Shi, Xuechen; Zhao, Tiankai; Huang, Changjin; Wei, Qiong; Tang, Xin; Santy, Lorraine C.; Saif, M Taher A; Zhang, Sulin.

In: Soft Matter, Vol. 15, No. 36, 01.01.2019, p. 7203-7210.

Research output: Contribution to journalArticle

Zhang, Y, Shi, X, Zhao, T, Huang, C, Wei, Q, Tang, X, Santy, LC, Saif, MTA & Zhang, S 2019, 'A traction force threshold signifies metastatic phenotypic change in multicellular epithelia', Soft Matter, vol. 15, no. 36, pp. 7203-7210. https://doi.org/10.1039/c9sm00733d
Zhang, Yao ; Shi, Xuechen ; Zhao, Tiankai ; Huang, Changjin ; Wei, Qiong ; Tang, Xin ; Santy, Lorraine C. ; Saif, M Taher A ; Zhang, Sulin. / A traction force threshold signifies metastatic phenotypic change in multicellular epithelia. In: Soft Matter. 2019 ; Vol. 15, No. 36. pp. 7203-7210.
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