A time course of orchestrated endophilin action in sensing, bending, and stabilizing curved membranes

Kumud R. Poudel, Yongming Dong, Hang Yu, Allen Su, Thuong Ho, Yan Liu, Klaus Schulten, Jihong Bai

Research output: Contribution to journalArticlepeer-review

Abstract

Numerous proteins act in concert to sculpt membrane compartments for cell signaling and metabolism. These proteins may act as curvature sensors, membrane benders, and scaffolding molecules. Here we show that endophilin, a critical protein for rapid endocytosis, quickly transforms from a curvature sensor into an active bender upon membrane association. We find that local membrane deformation does not occur until endophilin inserts its amphipathic helices into lipid bilayers, supporting an active bending mechanism through wedging. Our time-course studies show that endophilin continues to drive membrane changes on a seconds-to-minutes time scale, indicating that the duration of endocytosis events constrains the mode of endophilin action. Finally, we find a requirement of coordinated activities between wedging and scaffolding for endophilin to produce stable membrane tubules in vitro and to promote synaptic activity in vivo. Together these data demonstrate that endophilin is a multifaceted molecule that precisely integrates activities of sensing, bending, and stabilizing curvature to sculpt membranes with speed.

Original languageEnglish (US)
Pages (from-to)2119-2132
Number of pages14
JournalMolecular biology of the cell
Volume27
Issue number13
DOIs
StatePublished - Jul 1 2016

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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