A Target Restricted Assembly Method (TRAM) for Phylogenomics

Kevin Johnson; Kimberly K O Walden; Hugh Robertson

doi:10.1038/npre.2010.4612.1

A Target Restricted Assembly Method (TRAM) for Phylogenomics

Kevin Johnson, Kimberly K O Walden, Hugh Robertson

Research output: Contribution to journal › Article › peer-review

Abstract

While next generation sequencing technology can produce sequences covering the entire genome, assembly and annotation are still prohibitive steps for many phylogenomics applications. Here we describe a method of Target Restricted Assembly (TRAM) of a single lane of Illumina sequences for genes of relevance to phylogeny reconstruction, i.e. single copy protein-coding genes. This method has the potential to produce a data set of hundreds of genes using only one Illumina lane per taxon.

Original language	English (US)
Journal	Nature Precedings
DOIs	https://doi.org/10.1038/npre.2010.4612.1
State	Published - Jul 6 2010

Online availability

10.1038/npre.2010.4612.1

http://www.nature.com/articles/npre.2010.4612.1

Library availability

Discover UIUC Full Text

Cite this

@article{85b8789be50e4ef18119532e9ac77dac,

title = "A Target Restricted Assembly Method (TRAM) for Phylogenomics",

abstract = "While next generation sequencing technology can produce sequences covering the entire genome, assembly and annotation are still prohibitive steps for many phylogenomics applications. Here we describe a method of Target Restricted Assembly (TRAM) of a single lane of Illumina sequences for genes of relevance to phylogeny reconstruction, i.e. single copy protein-coding genes. This method has the potential to produce a data set of hundreds of genes using only one Illumina lane per taxon.",

author = "Kevin Johnson and Walden, {Kimberly K O} and Hugh Robertson",

year = "2010",

month = jul,

day = "6",

doi = "10.1038/npre.2010.4612.1",

language = "English (US)",

journal = "Nature Precedings",

issn = "1756-0357",

}

TY - JOUR

T1 - A Target Restricted Assembly Method (TRAM) for Phylogenomics

AU - Johnson, Kevin

AU - Walden, Kimberly K O

AU - Robertson, Hugh

PY - 2010/7/6

Y1 - 2010/7/6

N2 - While next generation sequencing technology can produce sequences covering the entire genome, assembly and annotation are still prohibitive steps for many phylogenomics applications. Here we describe a method of Target Restricted Assembly (TRAM) of a single lane of Illumina sequences for genes of relevance to phylogeny reconstruction, i.e. single copy protein-coding genes. This method has the potential to produce a data set of hundreds of genes using only one Illumina lane per taxon.

AB - While next generation sequencing technology can produce sequences covering the entire genome, assembly and annotation are still prohibitive steps for many phylogenomics applications. Here we describe a method of Target Restricted Assembly (TRAM) of a single lane of Illumina sequences for genes of relevance to phylogeny reconstruction, i.e. single copy protein-coding genes. This method has the potential to produce a data set of hundreds of genes using only one Illumina lane per taxon.

U2 - 10.1038/npre.2010.4612.1

DO - 10.1038/npre.2010.4612.1

M3 - Article

SN - 1756-0357

JO - Nature Precedings

JF - Nature Precedings

ER -

A Target Restricted Assembly Method (TRAM) for Phylogenomics

Abstract

Online availability

Library availability

Fingerprint

Cite this