@article{4f214fdefbfa45a6b46993fd286a3d59,
title = "A Target Recycling Amplification Process for the Digital Detection of Exosomal MicroRNAs through Photonic Resonator Absorption Microscopy",
abstract = "Exosomal microRNAs (miRNAs) have considerable potential as pivotal biomarkers to monitor cancer development, dis-ease progression, treatment effects and prognosis. Here, we report an efficient target recycling amplification process (TRAP) for the digital detection of miRNAs using photonic resonator absorption microscopy. We achieve multiplex digital detection with sub-attomolar sensitivity in 20 minutes, robust selectivity for single nucleotide variants, and a broad dynamic range from 1 aM to 1 pM. Compared with traditional qRT-PCR, TRAP showed similar accuracy in profiling exosomal miRNAs derived from cancer cells, but also exhibited at least 31-fold and 61-fold enhancement in the limits of miRNA-375 and miRNA-21 detection, respectively. The TRAP approach is ideal for exosomal or circulating miRNA biomarker quantification, where the miRNAs are present in low concentrations or sample volume, with potentials for frequent, low-cost, and minimally invasive point-of-care testing.",
keywords = "Amplification, Cancer Diagnostics, DNA Toehold Probe, Exosomal miRNA, Photonic Sensor",
author = "Xiaojing Wang and Skye Shepherd and Nantao Li and Congnyu Che and Tingjie Song and Yanyu Xiong and Palm, {Isabella Rose} and Bin Zhao and Manish Kohli and Utkan Demirci and Yi Lu and Cunningham, {Brian T.}",
note = "Funding Information: This work was supported by National Institutes of Health (R01EB029805). M.K. was supported by National Institutes of Health (R01-CA212097). N.L. acknowledges support from Zhejiang University ZJU-UIUC Joint Research Center (DREMES202001) and from the Mikashi award of the Carl R. Woese Institute for Genomic Biology. B. Z. acknowledges support from Genomic Diagnostics at the Woese Institute for Genomic Biology and the Grainger College of Engineering. Y.X. acknowledges support from the Cancer Center at Illinois C*STAR program. We thank Dr. Hui Xu and Huimin Zhang (Tumor Engineering and Phenotyping Facility, Cancer Center at Illinois, UIUC) for help with the experiments to extract exosomal miRNA from cell culture and qPCR quantification. Funding Information: This work was supported by National Institutes of Health (R01EB029805). M.K. was supported by National Institutes of Health (R01‐CA212097). N.L. acknowledges support from Zhejiang University ZJU‐UIUC Joint Research Center (DREMES202001) and from the Mikashi award of the Carl R. Woese Institute for Genomic Biology. B. Z. acknowledges support from Genomic Diagnostics at the Woese Institute for Genomic Biology and the Grainger College of Engineering. Y.X. acknowledges support from the Cancer Center at Illinois C*STAR program. We thank Dr. Hui Xu and Huimin Zhang (Tumor Engineering and Phenotyping Facility, Cancer Center at Illinois, UIUC) for help with the experiments to extract exosomal miRNA from cell culture and qPCR quantification. Publisher Copyright: {\textcopyright} 2023 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.",
year = "2023",
month = apr,
day = "11",
doi = "10.1002/anie.202217932",
language = "English (US)",
volume = "62",
journal = "Angewandte Chemie - International Edition",
issn = "1433-7851",
publisher = "John Wiley & Sons, Ltd.",
number = "16",
}