A surface forces study of DNA hybridization

Yoon Kyoun Cho; Sunhee Kim; Geunbae Lim; Steve Granick

doi:10.1021/la0113769

A surface forces study of DNA hybridization

Yoon Kyoun Cho, Sunhee Kim, Geunbae Lim, Steve Granick

Research output: Contribution to journal › Article › peer-review

Abstract

We report the first use of a surface forces apparatus to study the hybridization of DNA. We study the regime of very high ionic strength (≈1 M) at which commercial DNA chip operations are performed. Using a thiol end-attached single-stranded oligonucleotide, we find that exposure to the complementary strand resulted in larger thickness. In addition, the resistance to small-amplitude shear deformations when opposed to a nonadsorbing surface (mica) increased significantly after hybridization. The thickness at onset of significant shear resistance was, for single-stranded DNA, considerably less than that of hybridized DNA. This could provide a new method to detect DNA hybridization efficiency.

Original language	English (US)
Pages (from-to)	7732-7734
Number of pages	3
Journal	Langmuir
Volume	17
Issue number	25
DOIs	https://doi.org/10.1021/la0113769
State	Published - Dec 11 2001
Externally published	Yes

ASJC Scopus subject areas

General Materials Science
Condensed Matter Physics
Surfaces and Interfaces
Spectroscopy
Electrochemistry

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10.1021/la0113769

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N2 - We report the first use of a surface forces apparatus to study the hybridization of DNA. We study the regime of very high ionic strength (≈1 M) at which commercial DNA chip operations are performed. Using a thiol end-attached single-stranded oligonucleotide, we find that exposure to the complementary strand resulted in larger thickness. In addition, the resistance to small-amplitude shear deformations when opposed to a nonadsorbing surface (mica) increased significantly after hybridization. The thickness at onset of significant shear resistance was, for single-stranded DNA, considerably less than that of hybridized DNA. This could provide a new method to detect DNA hybridization efficiency.

AB - We report the first use of a surface forces apparatus to study the hybridization of DNA. We study the regime of very high ionic strength (≈1 M) at which commercial DNA chip operations are performed. Using a thiol end-attached single-stranded oligonucleotide, we find that exposure to the complementary strand resulted in larger thickness. In addition, the resistance to small-amplitude shear deformations when opposed to a nonadsorbing surface (mica) increased significantly after hybridization. The thickness at onset of significant shear resistance was, for single-stranded DNA, considerably less than that of hybridized DNA. This could provide a new method to detect DNA hybridization efficiency.

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A surface forces study of DNA hybridization

Abstract

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