A surface forces study of DNA hybridization

Yoon Kyoun Cho, Sunhee Kim, Geunbae Lim, Steve Granick

Research output: Contribution to journalArticlepeer-review


We report the first use of a surface forces apparatus to study the hybridization of DNA. We study the regime of very high ionic strength (≈1 M) at which commercial DNA chip operations are performed. Using a thiol end-attached single-stranded oligonucleotide, we find that exposure to the complementary strand resulted in larger thickness. In addition, the resistance to small-amplitude shear deformations when opposed to a nonadsorbing surface (mica) increased significantly after hybridization. The thickness at onset of significant shear resistance was, for single-stranded DNA, considerably less than that of hybridized DNA. This could provide a new method to detect DNA hybridization efficiency.

Original languageEnglish (US)
Pages (from-to)7732-7734
Number of pages3
Issue number25
StatePublished - Dec 11 2001

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry


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