A study of alterations in DNA epigenetic modifications (5mC and 5hmC) and gene expression influenced by simulated microgravity in human lymphoblastoid cells

Basudev Chowdhury, Arun Seetharam, Zhiping Wang, Yunlong Liu, Amy C. Lossie, Jyothi Thimmapuram, Joseph Irudayaraj

Research output: Contribution to journalArticle

Abstract

Cells alter their gene expression in response to exposure to various environmental changes. Epigenetic mechanisms such as DNA methylation are believed to regulate the alterations in gene expression patterns. In vitro and in vivo studies have documented changes in cellular proliferation, cytoskeletal remodeling, signal transduction, bone mineralization and immune deficiency under the influence of microgravity conditions experienced in space. However microgravity induced changes in the epigenome have not been well characterized. In this study we have used Next-generation Sequencing (NGS) to profile ground-based "simulated" microgravity induced changes on DNA methylation (5-methylcytosine or 5mC), hydroxymethylation (5-hydroxymethylcytosine or 5hmC), and simultaneous gene expression in cultured human lymphoblastoid cells. Our results indicate that simulated microgravity induced alterations in the methylome (∼60% of the differentially methylated regions or DMRs are hypomethylated and ∼92% of the differentially hydroxymethylated regions or DHMRs are hyperhydroxymethylated). Simulated microgravity also induced differential expression in 370 transcripts that were associated with crucial biological processes such as oxidative stress response, carbohydrate metabolism and regulation of transcription. While we were not able to obtain any global trend correlating the changes of methylation/ hydroxylation with gene expression, we have been able to profile the simulated microgravity induced changes of 5mC over some of the differentially expressed genes that includes five genes undergoing differential methylation over their promoters and twenty five genes undergoing differential methylation over their gene-bodies. To the best of our knowledge, this is the first NGS-based study to profile epigenomic patterns induced by short time exposure of simulated microgravity and we believe that our findings can be a valuable resource for future explorations.

Original languageEnglish (US)
Article numbere0147514
JournalPloS one
Volume11
Issue number1
DOIs
StatePublished - Jan 1 2016
Externally publishedYes

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microgravity
Weightlessness
Microgravity
Epigenomics
Gene expression
epigenetics
Gene Expression
gene expression
DNA
Methylation
Genes
methylation
cells
DNA methylation
DNA Methylation
genes
5-Methylcytosine
Biological Phenomena
Physiologic Calcification
bone mineralization

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

A study of alterations in DNA epigenetic modifications (5mC and 5hmC) and gene expression influenced by simulated microgravity in human lymphoblastoid cells. / Chowdhury, Basudev; Seetharam, Arun; Wang, Zhiping; Liu, Yunlong; Lossie, Amy C.; Thimmapuram, Jyothi; Irudayaraj, Joseph.

In: PloS one, Vol. 11, No. 1, e0147514, 01.01.2016.

Research output: Contribution to journalArticle

Chowdhury, Basudev ; Seetharam, Arun ; Wang, Zhiping ; Liu, Yunlong ; Lossie, Amy C. ; Thimmapuram, Jyothi ; Irudayaraj, Joseph. / A study of alterations in DNA epigenetic modifications (5mC and 5hmC) and gene expression influenced by simulated microgravity in human lymphoblastoid cells. In: PloS one. 2016 ; Vol. 11, No. 1.
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