One of the useful applications of chicken manure fermentation broth was foliar fertilization production. However, the effluent generated during foliar fertilization production processing was high in ammonia concentration, which was hard to be disposed by activated sludge method. Selecting microalgae to treat this kind of wastewater can realize pollutants purification, nutrients recovery and bio-energy production. A strain of Chlorella sp. was used. Results showed that the effluent can be used for microalgae cultivation. The optimal ammonia in the third (TS) and second step (SS) effluent for Chlorella sp. growth was around 500 mg/L and the net growth rate was 49.2 and 51.0 mg/L, respectively. The optimal addition amount of inorganic carbon was 228.6 mg/L for TS to cultivate Chlorella sp. By adding carbon resource every other day with trace elements, biomass reached to the highest value of 915.6 mg/L at 9 day. Without any addition of nutrient, the mixture of TS and SS can realize biomass accumulation of 497.0 mg/L. The highest removal of NH4 +-N could reach 40%.