A soybean cell wall protein is affected by seed color genotype

Jon T. Lindstrom, Lila O Vodkin

Research output: Contribution to journalArticle

Abstract

The dominant / gene inhibits accumulation of anthocyanin pigments in epidermal cells of the soybean seed coat. We compared saline-soluble proteins extracted from developing seed coats and identified a 35-kilodalton protein that was abundant in Richland (genotype I/I, yellow) and much reduced in an isogenic mutant line T157 (genotype i/i, imperfect black seed coats). We purified the 35-kilodalton protein by a novel procedure using chromatography on insoluble polyvinylpolypyrrolidone. The 35-kilodalton protein was composed primarily of proline, hydroxyproline, valine, tyrosine, and lysine. Three criteria (N-terminal amino acid sequence, amino acid composition, and sequence of a cDNA) proved that the seed coat 35-kilodalton protein was PRP1, a member of a proline-rich gene family expressed in hypocotyls and other soybean tissues. The levels of soluble PRP1 polypeptides and PRP1 mRNA were reduced in young seed coats with the recessive i/i genotype. These data demonstrated an unexpected and novel correlation between an anthocyanin gene and the quantitative levels of a specific, developmentally regulated cell wall protein. In contrast, PRP2, a closely related cell wall protein, was synthesized later in seed coat development and was not affected by the genotype of the I locus.

Original languageEnglish (US)
Pages (from-to)561-571
Number of pages11
JournalPlant Cell
Volume3
Issue number6
StatePublished - Jun 1991

Fingerprint

Soybeans
Cell Wall
Seed
Seeds
Color
Genotype
Cells
cell walls
soybeans
color
genotype
seeds
Proteins
proteins
Anthocyanins
Genes
Proline
proline
anthocyanins
Amino Acid Sequence

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

A soybean cell wall protein is affected by seed color genotype. / Lindstrom, Jon T.; Vodkin, Lila O.

In: Plant Cell, Vol. 3, No. 6, 06.1991, p. 561-571.

Research output: Contribution to journalArticle

Lindstrom, Jon T. ; Vodkin, Lila O. / A soybean cell wall protein is affected by seed color genotype. In: Plant Cell. 1991 ; Vol. 3, No. 6. pp. 561-571.
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