TY - JOUR
T1 - A small RNA promotes siderophore production through transcriptional and metabolic remodeling
AU - Salvail, Hubert
AU - Lanthier-Bourbonnais, Pascale
AU - Sobota, Jason Michael
AU - Caza, Mélissa
AU - Benjamin, Julie Anna M.
AU - Mendieta, Martha Eugènia Sequeira
AU - Lépine, François
AU - Dozois, Charles M.
AU - Imlay, James
AU - Massé, Eric
PY - 2010/8/24
Y1 - 2010/8/24
N2 - Siderophores are essential factors for iron (Fe) acquisition in bacteria during colonization and infection of eukaryotic hosts, which restrain iron access through iron-binding protein, such as lactoferrin and transferrin. The synthesis of siderophores by Escherichia coli is considered to be fully regulated at the transcriptional level by the Fe-responsive transcriptional repressor Fur. Here we characterized two different pathways that promote the production of the siderophore enterobactin via the action of the small RNA RyhB. First, RyhB is required for normal expression of an important enterobactin biosynthesis polycistron, entCEBAH. Second, RyhB directly represses the translation of cysE, which encodes a serine acetyltransferase that uses serine as a substrate for cysteine biosynthesis. Reduction of CysE activity by RyhB allows serine to be used as building blocks for enterobactin synthesis through the nonribosomal peptide synthesis pathway. Thus, RyhB plays an essential role in siderophore production and may modulate bacterial virulence through optimization of siderophore production.
AB - Siderophores are essential factors for iron (Fe) acquisition in bacteria during colonization and infection of eukaryotic hosts, which restrain iron access through iron-binding protein, such as lactoferrin and transferrin. The synthesis of siderophores by Escherichia coli is considered to be fully regulated at the transcriptional level by the Fe-responsive transcriptional repressor Fur. Here we characterized two different pathways that promote the production of the siderophore enterobactin via the action of the small RNA RyhB. First, RyhB is required for normal expression of an important enterobactin biosynthesis polycistron, entCEBAH. Second, RyhB directly represses the translation of cysE, which encodes a serine acetyltransferase that uses serine as a substrate for cysteine biosynthesis. Reduction of CysE activity by RyhB allows serine to be used as building blocks for enterobactin synthesis through the nonribosomal peptide synthesis pathway. Thus, RyhB plays an essential role in siderophore production and may modulate bacterial virulence through optimization of siderophore production.
KW - Enterobactin
KW - Iron
KW - RyhB
KW - SRNA
UR - http://www.scopus.com/inward/record.url?scp=77957015946&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77957015946&partnerID=8YFLogxK
U2 - 10.1073/pnas.1007805107
DO - 10.1073/pnas.1007805107
M3 - Article
C2 - 20696910
AN - SCOPUS:77957015946
SN - 0027-8424
VL - 107
SP - 15223
EP - 15228
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 34
ER -