A single-molecule view of genome editing proteins: Biophysical mechanisms for TALEs and CRISPR/Cas9

Luke Cuculis, Charles M. Schroeder

Research output: Contribution to journalReview article

Abstract

Exciting new advances in genome engineering have unlocked the potential to radically alter the treatment of human disease. In this review, we discuss the application of single-molecule techniques to uncover the mechanisms behind two premier classes of genome editing proteins: transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated system (Cas). These technologies have facilitated a striking number of gene editing applications in a variety of organisms; however, we are only beginning to understand the molecular mechanisms governing the DNA editing properties of these systems. Here, we discuss the DNA search and recognition process for TALEs and Cas9 that have been revealed by recent single-molecule experiments.

Original languageEnglish (US)
Pages (from-to)577-597
Number of pages21
JournalAnnual Review of Chemical and Biomolecular Engineering
Volume8
DOIs
StatePublished - Jun 7 2017

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Clustered Regularly Interspaced Short Palindromic Repeats
Genes
Proteins
Molecules
DNA
Transcription
Experiments

Keywords

  • CRISPR/Cas9
  • DNA search
  • Genome editing
  • Single molecule
  • TALEN

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)
  • Renewable Energy, Sustainability and the Environment

Cite this

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