A single amino acid converts a repressor to an activator of flowering

Yoshie Hanzawa, Tracy Money, Desmond Bradley

Research output: Contribution to journalArticle

Abstract

Homologous proteins occurring through gene duplication may give rise to novel functions through mutations affecting protein sequence or expression. Comparison of such homologues allows insight into how morphological traits evolve. However, it is often unclear which changes are key to determining new functions. To address these ideas, we have studied a system where two homologues have evolved clear and opposite functions in controlling a major developmental switch. In plants, flowering is a major developmental transition that is critical to reproductive success. Arabidopsis phosphatidylethanolamine-binding protein homologues TERMINAL FLOWER 1 (TFL1) and FLOWERING LOCUS T (FT) are key controllers of flowering, determining when and where flowers are made, but as opposing functions: TFL1 is a repressor, FT is an activator. We have uncovered a striking molecular basis for how these homologous proteins have diverged. Although <60% identical, we have shown that swapping a single amino acid is sufficient to convert TFL1 to FT function and vice versa. Therefore, these key residues may have strongly contributed to the selection of these important functions over plant evolution. Further, our results suggest that TFL1 and FT are highly conserved in biochemical function and that they act as repressors or activators of flowering through discrimination of structurally related interactors by a single residue.

Original languageEnglish (US)
Pages (from-to)7748-7753
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number21
DOIs
StatePublished - May 24 2005

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Amino Acids
Phosphatidylethanolamine Binding Protein
Proteins
Gene Duplication
Arabidopsis
Mutation

Keywords

  • FLOWERING LOCUS T
  • Phosphatidylethanolamine-binding protein
  • Raf-kinase inhibitor protein
  • TERMINAL FLOWER 1

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

A single amino acid converts a repressor to an activator of flowering. / Hanzawa, Yoshie; Money, Tracy; Bradley, Desmond.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, No. 21, 24.05.2005, p. 7748-7753.

Research output: Contribution to journalArticle

Hanzawa, Yoshie ; Money, Tracy ; Bradley, Desmond. / A single amino acid converts a repressor to an activator of flowering. In: Proceedings of the National Academy of Sciences of the United States of America. 2005 ; Vol. 102, No. 21. pp. 7748-7753.
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