A silicon nanopore device for on-chip patch clamp measurements of single ion channel activity

L. Plucinski, Y. Chen, G. L. Liu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Here we present a novel silicon nanopore planar patch clamp chip for single ion channel screening. We fabricate our devices using a combination of KOH and metal-assisted etching. Electrical characterization shows that the shunt capacitance and access resistance are within the accepted ranges for single channel recordings. In order to test our devices, we cultured and differentiated human neuroblastoma SH-SY5 Y cells on chip. We reliably obtained a high resistance seal to the cell membrane and report single ion channel activity recordings.

Original languageEnglish (US)
Title of host publicationMaterials and Concepts for Biomedical Sensing
EditorsL. Liu, E. Park, H. Schmidt, X. Fan
PublisherMaterials Research Society
Pages29-32
Number of pages4
ISBN (Electronic)9781510806054
DOIs
StatePublished - Jan 1 2015
Event2014 MRS Fall Meeting - Boston, United States
Duration: Nov 30 2014Dec 5 2014

Publication series

NameMaterials Research Society Symposium Proceedings
Volume1720
ISSN (Print)0272-9172

Other

Other2014 MRS Fall Meeting
CountryUnited States
CityBoston
Period11/30/1412/5/14

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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