A series of medium and high copy number arabinose-inducible Escherichia coli expression vectors compatible with pBR322 and pACYC184

Vandana Chakravartty, John E. Cronan

Research output: Contribution to journalArticle

Abstract

The original pBAD24 plasmid and the derived lower copy number (the pBAD322 series) expression vectors have been widely used in Escherichia coli, Salmonella enterica, and related bacteria. However, a flexible pBAD expression system has been available only in pMB1 (ColE1) vectors. We report a series of pBAD vectors that replicate using the origin of plasmid RSF1030 that are compatible with pMB1 (ColE1) and p15A (pACYC) vectors. Both high (≥. pBAD24) and medium (~. pBAD322) copy number plasmids encoding resistance to ampicillin, chloramphenicol, kanamycin, tetracycline, spectinomycin/streptomycin, gentamycin, or trimethoprim are available.

Original languageEnglish (US)
Pages (from-to)21-26
Number of pages6
JournalPlasmid
Volume81
DOIs
StatePublished - Sep 1 2015

Keywords

  • Copy number
  • Expression levels
  • PBAD24
  • PBR322
  • Plasmid Compatibility
  • RSF1030

ASJC Scopus subject areas

  • Molecular Biology

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