A series of medium and high copy number arabinose-inducible Escherichia coli expression vectors compatible with pBR322 and pACYC184

Vandana Chakravartty, John E. Cronan

Research output: Contribution to journalArticlepeer-review

Abstract

The original pBAD24 plasmid and the derived lower copy number (the pBAD322 series) expression vectors have been widely used in Escherichia coli, Salmonella enterica, and related bacteria. However, a flexible pBAD expression system has been available only in pMB1 (ColE1) vectors. We report a series of pBAD vectors that replicate using the origin of plasmid RSF1030 that are compatible with pMB1 (ColE1) and p15A (pACYC) vectors. Both high (≥. pBAD24) and medium (~. pBAD322) copy number plasmids encoding resistance to ampicillin, chloramphenicol, kanamycin, tetracycline, spectinomycin/streptomycin, gentamycin, or trimethoprim are available.

Original languageEnglish (US)
Pages (from-to)21-26
Number of pages6
JournalPlasmid
Volume81
DOIs
StatePublished - Sep 1 2015

Keywords

  • Copy number
  • Expression levels
  • PBAD24
  • PBR322
  • Plasmid Compatibility
  • RSF1030

ASJC Scopus subject areas

  • Molecular Biology

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