TY - JOUR
T1 - A sensitive and specific nanosensor for monitoring extracellular potassium levels in the brain
AU - Liu, Jianan
AU - Li, Fangyuan
AU - Wang, Yi
AU - Pan, Limin
AU - Lin, Peihua
AU - Zhang, Bo
AU - Zheng, Yanrong
AU - Xu, Yingwei
AU - Liao, Hongwei
AU - Ko, Giho
AU - Fei, Fan
AU - Xu, Cenglin
AU - Du, Yang
AU - Shin, Kwangsoo
AU - Kim, Dokyoon
AU - Jang, Sung Soo
AU - Chung, Hee Jung
AU - Tian, He
AU - Wang, Qi
AU - Guo, Wei
AU - Nam, Jwa Min
AU - Chen, Zhong
AU - Hyeon, Taeghwan
AU - Ling, Daishun
N1 - Publisher Copyright:
© 2020, The Author(s), under exclusive licence to Springer Nature Limited.
PY - 2020/4/1
Y1 - 2020/4/1
N2 - Extracellular potassium concentration affects the membrane potential of neurons, and, thus, neuronal activity. Indeed, alterations of potassium levels can be related to neurological disorders, such as epilepsy and Alzheimer’s disease, and, therefore, selectively detecting extracellular potassium would allow the monitoring of disease. However, currently available optical reporters are not capable of detecting small changes in potassium, in particular, in freely moving animals. Furthermore, they are susceptible to interference from sodium ions. Here, we report a highly sensitive and specific potassium nanosensor that can monitor potassium changes in the brain of freely moving mice undergoing epileptic seizures. An optical potassium indicator is embedded in mesoporous silica nanoparticles, which are shielded by an ultrathin layer of a potassium-permeable membrane, which prevents diffusion of other cations and allows the specific capturing of potassium ions. The shielded nanosensor enables the spatial mapping of potassium ion release in the hippocampus of freely moving mice.
AB - Extracellular potassium concentration affects the membrane potential of neurons, and, thus, neuronal activity. Indeed, alterations of potassium levels can be related to neurological disorders, such as epilepsy and Alzheimer’s disease, and, therefore, selectively detecting extracellular potassium would allow the monitoring of disease. However, currently available optical reporters are not capable of detecting small changes in potassium, in particular, in freely moving animals. Furthermore, they are susceptible to interference from sodium ions. Here, we report a highly sensitive and specific potassium nanosensor that can monitor potassium changes in the brain of freely moving mice undergoing epileptic seizures. An optical potassium indicator is embedded in mesoporous silica nanoparticles, which are shielded by an ultrathin layer of a potassium-permeable membrane, which prevents diffusion of other cations and allows the specific capturing of potassium ions. The shielded nanosensor enables the spatial mapping of potassium ion release in the hippocampus of freely moving mice.
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U2 - 10.1038/s41565-020-0634-4
DO - 10.1038/s41565-020-0634-4
M3 - Article
C2 - 32042163
AN - SCOPUS:85079435264
SN - 1748-3387
VL - 15
SP - 321
EP - 330
JO - Nature Nanotechnology
JF - Nature Nanotechnology
IS - 4
ER -