A rule of seven in Watson-Crick base-pairing of mismatched sequences

Ibrahim I. Cisse, Hajin Kim, Taekjip Ha

Research output: Contribution to journalArticlepeer-review

Abstract

Sequence recognition through base-pairing is essential for DNA repair and gene regulation, but the basic rules governing this process remain elusive. In particular, the kinetics of annealing between two imperfectly matched strands is not well characterized, despite its potential importance in nucleic acid-based biotechnologies and gene silencing. Here we use single-molecule fluorescence to visualize the multiple annealing and melting reactions of two untethered strands inside a porous vesicle, allowing us to precisely quantify the annealing and melting rates. The data as a function of mismatch position suggest that seven contiguous base pairs are needed for rapid annealing of DNA and RNA. This phenomenological rule of seven may underlie the requirement for seven nucleotides of complementarity to seed gene silencing by small noncoding RNA and may help guide performance improvement in DNA- and RNA-based bio- and nanotechnologies, in which off-target effects can be detrimental.

Original languageEnglish (US)
Pages (from-to)623-627
Number of pages5
JournalNature Structural and Molecular Biology
Volume19
Issue number6
DOIs
StatePublished - Jun 2012

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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