@article{81e52f2addaf4db08b64a76c260cabaf,
title = "A RubisCO-like protein links SAM metabolism with isoprenoid biosynthesis",
abstract = "Functional assignment of uncharacterized proteins is a challenge in the era of large-scale genome sequencing. Here, we combine in extracto NMR, proteomics and transcriptomics with a newly developed (knock-out) metabolomics platform to determine a potential physiological role for a ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO)-like protein from Rhodospirillum rubrum. Our studies unraveled an unexpected link in bacterial central carbon metabolism between S-adenosylmethionine-dependent polyamine metabolism and isoprenoid biosynthesis and also provide an alternative approach to assign enzyme function at the organismic level.",
author = "Erb, {Tobias J.} and Evans, {Bradley S.} and Kyuil Cho and Warlick, {Benjamin P.} and Jaya Sriram and Wood, {B. Mc Kay} and Imker, {Heidi J.} and Sweedler, {Jonathan V.} and Tabita, {F. Robert} and Gerlt, {John A.}",
note = "Funding Information: This research was supported by a fellowship to T.J.E. from the Deutsche Forschungsgemeinschaft (ER 646/1-1) and research grants from the US National Institutes of Health (R01GM065155, R01GM095742, P01GM071790 and U54GM093342). We would like to thank J.E. Cronan for critical comments and C. Deane and T. Miyairi for excellent technical assistance. We also acknowledge S.C. Almo and B.S. Hillerich (New York Structural Genomics Research Consortium) for providing expression plasmids and purified cupin for in vitro assays.",
year = "2012",
month = nov,
doi = "10.1038/nchembio.1087",
language = "English (US)",
volume = "8",
pages = "926--932",
journal = "Nature chemical biology",
issn = "1552-4450",
publisher = "Nature Research",
number = "11",
}