A RubisCO-like protein links SAM metabolism with isoprenoid biosynthesis

Tobias J. Erb, Bradley S. Evans, Kyuil Cho, Benjamin P. Warlick, Jaya Sriram, B. Mc Kay Wood, Heidi J Imker, Jonathan V Sweedler, F. Robert Tabita, John Alan Gerlt

Research output: Contribution to journalArticle

Abstract

Functional assignment of uncharacterized proteins is a challenge in the era of large-scale genome sequencing. Here, we combine in extracto NMR, proteomics and transcriptomics with a newly developed (knock-out) metabolomics platform to determine a potential physiological role for a ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO)-like protein from Rhodospirillum rubrum. Our studies unraveled an unexpected link in bacterial central carbon metabolism between S-adenosylmethionine-dependent polyamine metabolism and isoprenoid biosynthesis and also provide an alternative approach to assign enzyme function at the organismic level.

Original languageEnglish (US)
Pages (from-to)926-932
Number of pages7
JournalNature chemical biology
Volume8
Issue number11
DOIs
StatePublished - Jan 1 2012

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Oxygenases
Terpenes
Rhodospirillum rubrum
S-Adenosylmethionine
Metabolomics
Polyamines
Proteomics
Proteins
Carbon
Genome
Enzymes
ribulose-1,5 diphosphate
link protein

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Erb, T. J., Evans, B. S., Cho, K., Warlick, B. P., Sriram, J., Wood, B. M. K., ... Gerlt, J. A. (2012). A RubisCO-like protein links SAM metabolism with isoprenoid biosynthesis. Nature chemical biology, 8(11), 926-932. https://doi.org/10.1038/nchembio.1087

A RubisCO-like protein links SAM metabolism with isoprenoid biosynthesis. / Erb, Tobias J.; Evans, Bradley S.; Cho, Kyuil; Warlick, Benjamin P.; Sriram, Jaya; Wood, B. Mc Kay; Imker, Heidi J; Sweedler, Jonathan V; Tabita, F. Robert; Gerlt, John Alan.

In: Nature chemical biology, Vol. 8, No. 11, 01.01.2012, p. 926-932.

Research output: Contribution to journalArticle

Erb, TJ, Evans, BS, Cho, K, Warlick, BP, Sriram, J, Wood, BMK, Imker, HJ, Sweedler, JV, Tabita, FR & Gerlt, JA 2012, 'A RubisCO-like protein links SAM metabolism with isoprenoid biosynthesis', Nature chemical biology, vol. 8, no. 11, pp. 926-932. https://doi.org/10.1038/nchembio.1087
Erb TJ, Evans BS, Cho K, Warlick BP, Sriram J, Wood BMK et al. A RubisCO-like protein links SAM metabolism with isoprenoid biosynthesis. Nature chemical biology. 2012 Jan 1;8(11):926-932. https://doi.org/10.1038/nchembio.1087
Erb, Tobias J. ; Evans, Bradley S. ; Cho, Kyuil ; Warlick, Benjamin P. ; Sriram, Jaya ; Wood, B. Mc Kay ; Imker, Heidi J ; Sweedler, Jonathan V ; Tabita, F. Robert ; Gerlt, John Alan. / A RubisCO-like protein links SAM metabolism with isoprenoid biosynthesis. In: Nature chemical biology. 2012 ; Vol. 8, No. 11. pp. 926-932.
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