A RT-PCR assay for the detection of encephalomyocarditis virus infections in pigs

Lester J. Pérez, Heidy Díaz de Arce

Research output: Contribution to journalArticle

Abstract

Encephalomyocarditis virus (EMCV) infections can cause losses in pig farms all over the world. Rapid, sensitive and unequivocal detection of this virus is therefore essential for the diagnosis and control of the disease. An RT-PCR assay was developed, optimized and evaluated for encephalomyocarditis virus detection in organ based on a pair of primers that amplifies a 165 bp DNA fragment from a highly conserved nucleotide region of the viral 3D glycoprotein. PCR products of the expected size were obtained from Cuban EMCV 744/03 strain. Non-specific reactions were not observed when other porcine RNA genome viruses and uninfected cells were used. The analytical sensitivity of the test was estimated to be 2 TCID50/50 μL. The analysis of tissue homogenate samples from naturally infected animals proved the potential usefulness of the method for a rapid disease diagnosis from field cases.

Original languageEnglish (US)
Pages (from-to)988-993
Number of pages6
JournalBrazilian Journal of Microbiology
Volume40
Issue number4
DOIs
StatePublished - Jan 1 2009
Externally publishedYes

Keywords

  • Encephalomyocarditis virus
  • Pig
  • RT-PCR assay

ASJC Scopus subject areas

  • Microbiology

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