A Ratiometric Acoustogenic Probe for in Vivo Imaging of Endogenous Nitric Oxide

Christopher J. Reinhardt, Effie Y. Zhou, Michael D. Jorgensen, Gina Partipilo, Jefferson Kar Fai Chan

Research output: Contribution to journalArticle

Abstract

Photoacoustic (PA) imaging is an emerging imaging modality that utilizes optical excitation and acoustic detection to enable high resolution at centimeter depths. The development of activatable PA probes can expand the utility of this technology to allow for detection of specific stimuli within live-animal models. Herein, we report the design, development, and evaluation of a series of Acoustogenic Probe(s) for Nitric Oxide (APNO) for the ratiometric, analyte-specific detection of nitric oxide (NO) in vivo. The best probe in the series, APNO-5, rapidly responds to NO to form an N-nitroso product with a concomitant 91 nm hypsochromic shift. This property enables ratiometric PA imaging upon selective irradiation of APNO-5 and the corresponding product, tAPNO-5. Moreover, APNO-5 displays the requisite photophysical characteristics for in vivo PA imaging (e.g., high absorptivity, low quantum yield) as well as high biocompatibility, stability, and selectivity for NO over a variety of biologically relevant analytes. APNO-5 was successfully applied to the detection of endogenous NO in a murine lipopolysaccharide-induced inflammation model. Our studies show a 1.9-fold increase in PA signal at 680 nm and a 1.3-fold ratiometric turn-on relative to a saline control.

Original languageEnglish (US)
Pages (from-to)1011-1018
Number of pages8
JournalJournal of the American Chemical Society
Volume140
Issue number3
DOIs
StatePublished - Jan 24 2018

Fingerprint

Photoacoustic effect
Nitric oxide
nitric oxide
Nitric Oxide
probe
Imaging techniques
fold
Photoexcitation
Quantum yield
Biocompatibility
Acoustics
Lipopolysaccharides
irradiation
Animals
acoustics
Animal Models
Irradiation
Inflammation
Technology
detection

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

A Ratiometric Acoustogenic Probe for in Vivo Imaging of Endogenous Nitric Oxide. / Reinhardt, Christopher J.; Zhou, Effie Y.; Jorgensen, Michael D.; Partipilo, Gina; Chan, Jefferson Kar Fai.

In: Journal of the American Chemical Society, Vol. 140, No. 3, 24.01.2018, p. 1011-1018.

Research output: Contribution to journalArticle

Reinhardt, Christopher J. ; Zhou, Effie Y. ; Jorgensen, Michael D. ; Partipilo, Gina ; Chan, Jefferson Kar Fai. / A Ratiometric Acoustogenic Probe for in Vivo Imaging of Endogenous Nitric Oxide. In: Journal of the American Chemical Society. 2018 ; Vol. 140, No. 3. pp. 1011-1018.
@article{0e2c3de602a4416081afc1bddfd7996e,
title = "A Ratiometric Acoustogenic Probe for in Vivo Imaging of Endogenous Nitric Oxide",
abstract = "Photoacoustic (PA) imaging is an emerging imaging modality that utilizes optical excitation and acoustic detection to enable high resolution at centimeter depths. The development of activatable PA probes can expand the utility of this technology to allow for detection of specific stimuli within live-animal models. Herein, we report the design, development, and evaluation of a series of Acoustogenic Probe(s) for Nitric Oxide (APNO) for the ratiometric, analyte-specific detection of nitric oxide (NO) in vivo. The best probe in the series, APNO-5, rapidly responds to NO to form an N-nitroso product with a concomitant 91 nm hypsochromic shift. This property enables ratiometric PA imaging upon selective irradiation of APNO-5 and the corresponding product, tAPNO-5. Moreover, APNO-5 displays the requisite photophysical characteristics for in vivo PA imaging (e.g., high absorptivity, low quantum yield) as well as high biocompatibility, stability, and selectivity for NO over a variety of biologically relevant analytes. APNO-5 was successfully applied to the detection of endogenous NO in a murine lipopolysaccharide-induced inflammation model. Our studies show a 1.9-fold increase in PA signal at 680 nm and a 1.3-fold ratiometric turn-on relative to a saline control.",
author = "Reinhardt, {Christopher J.} and Zhou, {Effie Y.} and Jorgensen, {Michael D.} and Gina Partipilo and Chan, {Jefferson Kar Fai}",
year = "2018",
month = "1",
day = "24",
doi = "10.1021/jacs.7b10783",
language = "English (US)",
volume = "140",
pages = "1011--1018",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "American Chemical Society",
number = "3",

}

TY - JOUR

T1 - A Ratiometric Acoustogenic Probe for in Vivo Imaging of Endogenous Nitric Oxide

AU - Reinhardt, Christopher J.

AU - Zhou, Effie Y.

AU - Jorgensen, Michael D.

AU - Partipilo, Gina

AU - Chan, Jefferson Kar Fai

PY - 2018/1/24

Y1 - 2018/1/24

N2 - Photoacoustic (PA) imaging is an emerging imaging modality that utilizes optical excitation and acoustic detection to enable high resolution at centimeter depths. The development of activatable PA probes can expand the utility of this technology to allow for detection of specific stimuli within live-animal models. Herein, we report the design, development, and evaluation of a series of Acoustogenic Probe(s) for Nitric Oxide (APNO) for the ratiometric, analyte-specific detection of nitric oxide (NO) in vivo. The best probe in the series, APNO-5, rapidly responds to NO to form an N-nitroso product with a concomitant 91 nm hypsochromic shift. This property enables ratiometric PA imaging upon selective irradiation of APNO-5 and the corresponding product, tAPNO-5. Moreover, APNO-5 displays the requisite photophysical characteristics for in vivo PA imaging (e.g., high absorptivity, low quantum yield) as well as high biocompatibility, stability, and selectivity for NO over a variety of biologically relevant analytes. APNO-5 was successfully applied to the detection of endogenous NO in a murine lipopolysaccharide-induced inflammation model. Our studies show a 1.9-fold increase in PA signal at 680 nm and a 1.3-fold ratiometric turn-on relative to a saline control.

AB - Photoacoustic (PA) imaging is an emerging imaging modality that utilizes optical excitation and acoustic detection to enable high resolution at centimeter depths. The development of activatable PA probes can expand the utility of this technology to allow for detection of specific stimuli within live-animal models. Herein, we report the design, development, and evaluation of a series of Acoustogenic Probe(s) for Nitric Oxide (APNO) for the ratiometric, analyte-specific detection of nitric oxide (NO) in vivo. The best probe in the series, APNO-5, rapidly responds to NO to form an N-nitroso product with a concomitant 91 nm hypsochromic shift. This property enables ratiometric PA imaging upon selective irradiation of APNO-5 and the corresponding product, tAPNO-5. Moreover, APNO-5 displays the requisite photophysical characteristics for in vivo PA imaging (e.g., high absorptivity, low quantum yield) as well as high biocompatibility, stability, and selectivity for NO over a variety of biologically relevant analytes. APNO-5 was successfully applied to the detection of endogenous NO in a murine lipopolysaccharide-induced inflammation model. Our studies show a 1.9-fold increase in PA signal at 680 nm and a 1.3-fold ratiometric turn-on relative to a saline control.

UR - http://www.scopus.com/inward/record.url?scp=85041126234&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85041126234&partnerID=8YFLogxK

U2 - 10.1021/jacs.7b10783

DO - 10.1021/jacs.7b10783

M3 - Article

C2 - 29313677

AN - SCOPUS:85041126234

VL - 140

SP - 1011

EP - 1018

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

IS - 3

ER -