A rat liver RNA-DNA-protein complex with template activity for protein synthesis

D. H. Roe, N. G. Avadhani, D. E. Buetow

Research output: Contribution to journalArticle

Abstract

Total RNA isolated from rat liver by a phenol technique at pH 9.0 has template activity for protein synthesis. Much of this template activity is extracted with 1.0 M NaCl. An RNA-DNA-protein complex with template activity is isolated from the saline extract by precipitation with isopropanol. The complex, composed of 81-89 % RNA, 5-12 % DNA, and 6-7 % protein, shows the highest specific template activity for protein synthesis of all the liver RNA fractions tested. Streptomycin and ribonuclease reduce the template activity shown by the complex as well as that shown by the total RNA and the saline-insoluble RNA.

Original languageEnglish (US)
Pages (from-to)430-441
Number of pages12
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume246
Issue number3
DOIs
StatePublished - Sep 24 1971

ASJC Scopus subject areas

  • Medicine(all)

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