A rat liver RNA-DNA-protein complex with template activity for protein synthesis

D. H. Roe; N. G. Avadhani; D. E. Buetow

doi:10.1016/0005-2787(71)90779-9

A rat liver RNA-DNA-protein complex with template activity for protein synthesis

D. H. Roe, N. G. Avadhani, D. E. Buetow

Molecular and Integrative Physiology

Research output: Contribution to journal › Article › peer-review

Abstract

Total RNA isolated from rat liver by a phenol technique at pH 9.0 has template activity for protein synthesis. Much of this template activity is extracted with 1.0 M NaCl. An RNA-DNA-protein complex with template activity is isolated from the saline extract by precipitation with isopropanol. The complex, composed of 81-89 % RNA, 5-12 % DNA, and 6-7 % protein, shows the highest specific template activity for protein synthesis of all the liver RNA fractions tested. Streptomycin and ribonuclease reduce the template activity shown by the complex as well as that shown by the total RNA and the saline-insoluble RNA.

Original language	English (US)
Pages (from-to)	430-441
Number of pages	12
Journal	BBA Section Nucleic Acids And Protein Synthesis
Volume	246
Issue number	3
DOIs	https://doi.org/10.1016/0005-2787(71)90779-9
State	Published - Sep 24 1971

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Medicine(all)

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10.1016/0005-2787(71)90779-9

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title = "A rat liver RNA-DNA-protein complex with template activity for protein synthesis",

abstract = "Total RNA isolated from rat liver by a phenol technique at pH 9.0 has template activity for protein synthesis. Much of this template activity is extracted with 1.0 M NaCl. An RNA-DNA-protein complex with template activity is isolated from the saline extract by precipitation with isopropanol. The complex, composed of 81-89 % RNA, 5-12 % DNA, and 6-7 % protein, shows the highest specific template activity for protein synthesis of all the liver RNA fractions tested. Streptomycin and ribonuclease reduce the template activity shown by the complex as well as that shown by the total RNA and the saline-insoluble RNA.",

author = "Roe, {D. H.} and Avadhani, {N. G.} and Buetow, {D. E.}",

note = "Funding Information: This research was supported by grant HD 03163 from the National Institute of Child Health and Human Development.",

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T1 - A rat liver RNA-DNA-protein complex with template activity for protein synthesis

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AU - Buetow, D. E.

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N2 - Total RNA isolated from rat liver by a phenol technique at pH 9.0 has template activity for protein synthesis. Much of this template activity is extracted with 1.0 M NaCl. An RNA-DNA-protein complex with template activity is isolated from the saline extract by precipitation with isopropanol. The complex, composed of 81-89 % RNA, 5-12 % DNA, and 6-7 % protein, shows the highest specific template activity for protein synthesis of all the liver RNA fractions tested. Streptomycin and ribonuclease reduce the template activity shown by the complex as well as that shown by the total RNA and the saline-insoluble RNA.

AB - Total RNA isolated from rat liver by a phenol technique at pH 9.0 has template activity for protein synthesis. Much of this template activity is extracted with 1.0 M NaCl. An RNA-DNA-protein complex with template activity is isolated from the saline extract by precipitation with isopropanol. The complex, composed of 81-89 % RNA, 5-12 % DNA, and 6-7 % protein, shows the highest specific template activity for protein synthesis of all the liver RNA fractions tested. Streptomycin and ribonuclease reduce the template activity shown by the complex as well as that shown by the total RNA and the saline-insoluble RNA.

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A rat liver RNA-DNA-protein complex with template activity for protein synthesis

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