Total RNA isolated from rat liver by a phenol technique at pH 9.0 has template activity for protein synthesis. Much of this template activity is extracted with 1.0 M NaCl. An RNA-DNA-protein complex with template activity is isolated from the saline extract by precipitation with isopropanol. The complex, composed of 81-89 % RNA, 5-12 % DNA, and 6-7 % protein, shows the highest specific template activity for protein synthesis of all the liver RNA fractions tested. Streptomycin and ribonuclease reduce the template activity shown by the complex as well as that shown by the total RNA and the saline-insoluble RNA.
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