A rapid chemiluminescent detection method for barley yellow dwarf virus

Hanafy M. Fouly, Leslie L Domier, Cleora J. D'Arcy

Research output: Contribution to journalArticle

Abstract

Barley yellow dwarf virus (BYDV-PAV-IL) was detected with biotinylated in vitro transcript cDNA using a chemiluminescent substrate on nylon membranes. Signals were detected on X-ray film and quantified using either a densitometer or an ELISA plate reader. The time required for sample preparation was reduced so that the entire protocol could be completed in two days. The in vitro transcript probes could detect 1 ng of purified virus and as little as 1 μl of sap extracts prepared from infected oat shoots.

Original languageEnglish (US)
Pages (from-to)291-298
Number of pages8
JournalJournal of Virological Methods
Volume39
Issue number3
DOIs
StatePublished - Sep 1992

Keywords

  • Dot blot
  • Hybridization
  • Luteovirus

ASJC Scopus subject areas

  • Virology

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