A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis

Guanhua Xun; Stephan Thomas Lane; Vassily Andrew Petrov; Brandon Elliott Pepa; Huimin Zhao

doi:10.1038/s41467-021-23185-x

A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis

Guanhua Xun, Stephan Thomas Lane, Vassily Andrew Petrov, Brandon Elliott Pepa, Huimin Zhao

Research output: Contribution to journal › Article › peer-review

Abstract

The need for rapid, accurate, and scalable testing systems for COVID-19 diagnosis is clear and urgent. Here, we report a rapid Scalable and Portable Testing (SPOT) system consisting of a rapid, highly sensitive, and accurate assay and a battery-powered portable device for COVID-19 diagnosis. The SPOT assay comprises a one-pot reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) followed by PfAgo-based target sequence detection. It is capable of detecting the N gene and E gene in a multiplexed reaction with the limit of detection (LoD) of 0.44 copies/μL and 1.09 copies/μL, respectively, in SARS-CoV-2 virus-spiked saliva samples within 30 min. Moreover, the SPOT system is used to analyze 104 clinical saliva samples and identified 28/30 (93.3% sensitivity) SARS-CoV-2 positive samples (100% sensitivity if LoD is considered) and 73/74 (98.6% specificity) SARS-CoV-2 negative samples. This combination of speed, accuracy, sensitivity, and portability will enable high-volume, low-cost access to areas in need of urgent COVID-19 testing capabilities.

Original language	English (US)
Article number	2905
Journal	Nature communications
Volume	12
Issue number	1
DOIs	https://doi.org/10.1038/s41467-021-23185-x
State	Published - Dec 2021

Keywords

COVID-19
severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Pandemic
Coronavirus
Novel coronavirus
2019-nCoV

ASJC Scopus subject areas

General Physics and Astronomy
General Chemistry
General Biochemistry, Genetics and Molecular Biology

Online availability

10.1038/s41467-021-23185-xLicense: CC BY

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title = "A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis",

abstract = "The need for rapid, accurate, and scalable testing systems for COVID-19 diagnosis is clear and urgent. Here, we report a rapid Scalable and Portable Testing (SPOT) system consisting of a rapid, highly sensitive, and accurate assay and a battery-powered portable device for COVID-19 diagnosis. The SPOT assay comprises a one-pot reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) followed by PfAgo-based target sequence detection. It is capable of detecting the N gene and E gene in a multiplexed reaction with the limit of detection (LoD) of 0.44 copies/μL and 1.09 copies/μL, respectively, in SARS-CoV-2 virus-spiked saliva samples within 30 min. Moreover, the SPOT system is used to analyze 104 clinical saliva samples and identified 28/30 (93.3% sensitivity) SARS-CoV-2 positive samples (100% sensitivity if LoD is considered) and 73/74 (98.6% specificity) SARS-CoV-2 negative samples. This combination of speed, accuracy, sensitivity, and portability will enable high-volume, low-cost access to areas in need of urgent COVID-19 testing capabilities.",

keywords = "COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Pandemic, Coronavirus, Novel coronavirus, 2019-nCoV",

author = "Guanhua Xun and Lane, {Stephan Thomas} and Petrov, {Vassily Andrew} and Pepa, {Brandon Elliott} and Huimin Zhao",

note = "Funding Information: This work was supported by Steve L. Miller Endowed Chair Fund and Carl R. Woese Institute for Genomic Biology at University of Illinois at Urbana-Champaign. The clinical saliva samples were obtained from University of Illinois at Urbana-Champaign COVID-19 Registry and we thank Dr. Leyi Wang for preparing these samples. We thank the University of Illinois at Urbana-Champaign Aerospace and Physics-MRL machine shops for assistance in creating the heat block used in the SPOT device. Publisher Copyright: {\textcopyright} 2021, The Author(s).",

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doi = "10.1038/s41467-021-23185-x",

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AU - Zhao, Huimin

N1 - Funding Information: This work was supported by Steve L. Miller Endowed Chair Fund and Carl R. Woese Institute for Genomic Biology at University of Illinois at Urbana-Champaign. The clinical saliva samples were obtained from University of Illinois at Urbana-Champaign COVID-19 Registry and we thank Dr. Leyi Wang for preparing these samples. We thank the University of Illinois at Urbana-Champaign Aerospace and Physics-MRL machine shops for assistance in creating the heat block used in the SPOT device. Publisher Copyright: © 2021, The Author(s).

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A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis

Abstract

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Portable, affordable, accurate, fast: Team invents new COVID-19 test

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A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis

Abstract

Keywords

ASJC Scopus subject areas

Online availability

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Portable, affordable, accurate, fast: Team invents new COVID-19 test

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