A quantitative radioimmunological screening method for specific gene products

Robert G. Kranz; Robert B. Gennis

doi:10.1016/0003-2697(82)90169-5

A quantitative radioimmunological screening method for specific gene products

Robert G. Kranz, Robert B. Gennis

Biochemistry

Research output: Contribution to journal › Article › peer-review

Abstract

A rapid, sensitive, and quantitative radioimmunoassay for specific translation products was developed using Escherichia coli cells grown in 96-well microtiter plates. A simple and inexpensive apparatus that facilitates the simultaneous transfer of all 96 detergent-lysed cultures to nitrocellulose within 30 s is described. Following this quantitative transfer, selected proteins are screened using specific antisera and ¹²⁵I-Protein A. The technique works with either cytoplasmic or membrane proteins. As little as a two- to threefold increase of a gene product over normal levels can easily be detected.

Original language	English (US)
Pages (from-to)	247-257
Number of pages	11
Journal	Analytical Biochemistry
Volume	127
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(82)90169-5
State	Published - Dec 1982

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Online availability

10.1016/0003-2697(82)90169-5

Library availability

Discover UIUC Full Text

Cite this

@article{f2cb2f929836443cbdd1e2a6974b1cdf,

title = "A quantitative radioimmunological screening method for specific gene products",

abstract = "A rapid, sensitive, and quantitative radioimmunoassay for specific translation products was developed using Escherichia coli cells grown in 96-well microtiter plates. A simple and inexpensive apparatus that facilitates the simultaneous transfer of all 96 detergent-lysed cultures to nitrocellulose within 30 s is described. Following this quantitative transfer, selected proteins are screened using specific antisera and 125I-Protein A. The technique works with either cytoplasmic or membrane proteins. As little as a two- to threefold increase of a gene product over normal levels can easily be detected.",

author = "Kranz, {Robert G.} and Gennis, {Robert B.}",

year = "1982",

month = dec,

doi = "10.1016/0003-2697(82)90169-5",

language = "English (US)",

volume = "127",

pages = "247--257",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - A quantitative radioimmunological screening method for specific gene products

AU - Kranz, Robert G.

AU - Gennis, Robert B.

PY - 1982/12

Y1 - 1982/12

N2 - A rapid, sensitive, and quantitative radioimmunoassay for specific translation products was developed using Escherichia coli cells grown in 96-well microtiter plates. A simple and inexpensive apparatus that facilitates the simultaneous transfer of all 96 detergent-lysed cultures to nitrocellulose within 30 s is described. Following this quantitative transfer, selected proteins are screened using specific antisera and 125I-Protein A. The technique works with either cytoplasmic or membrane proteins. As little as a two- to threefold increase of a gene product over normal levels can easily be detected.

AB - A rapid, sensitive, and quantitative radioimmunoassay for specific translation products was developed using Escherichia coli cells grown in 96-well microtiter plates. A simple and inexpensive apparatus that facilitates the simultaneous transfer of all 96 detergent-lysed cultures to nitrocellulose within 30 s is described. Following this quantitative transfer, selected proteins are screened using specific antisera and 125I-Protein A. The technique works with either cytoplasmic or membrane proteins. As little as a two- to threefold increase of a gene product over normal levels can easily be detected.

UR - http://www.scopus.com/inward/record.url?scp=0020349277&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020349277&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(82)90169-5

DO - 10.1016/0003-2697(82)90169-5

M3 - Article

C2 - 6762108

AN - SCOPUS:0020349277

SN - 0003-2697

VL - 127

SP - 247

EP - 257

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

A quantitative radioimmunological screening method for specific gene products

Abstract

ASJC Scopus subject areas

Online availability

Library availability

Related links

Fingerprint

Cite this