A quantitative assay for Amaranthus palmeri identification

Brent P. Murphy; Diane E. Plewa; Elizabeth Phillippi; Suzanne M. Bissonnette; Patrick J. Tranel

doi:10.1002/ps.4632

A quantitative assay for Amaranthus palmeri identification

Brent P. Murphy, Diane E. Plewa, Elizabeth Phillippi, Suzanne M. Bissonnette, Patrick J. Tranel

Crop Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

BACKGROUND: Amaranthus palmeri recently has been brought into the Midwestern USA as a contaminant in Conservation Reserve Program seeding mixes. Rapid species screening is required to mitigate the risk of continued species movement. RESULTS: Markers were developed for A. palmeri-specific nucleotide polymorphisms in the internal transcribed spacer of the ribosomal coding region. A quantitative polymerase chain reaction (qPCR) assay successfully identified A. palmeri from single-plant samples, simulated mixed-plant samples and seed mixtures. CONCLUSION: A qPCR assay for distinguishing A. palmeri from 12 other Amaranthus spp. was developed and validated. The assay can consistently detect a single A. palmeri seed when present in a pool of 100 total Amaranthus spp. seeds.

Original language	English (US)
Pages (from-to)	2221-2224
Number of pages	4
Journal	Pest Management Science
Volume	73
Issue number	11
DOIs	https://doi.org/10.1002/ps.4632
State	Published - Nov 2017

Keywords

Amaranthus palmeri
Conservation Reserve Program
qPCR
species identification

ASJC Scopus subject areas

Agronomy and Crop Science
Insect Science

Online availability

10.1002/ps.4632

Library availability

Discover UIUC Full Text

Cite this

@article{d038d860a98849538fb5e20c1531f716,

title = "A quantitative assay for Amaranthus palmeri identification",

abstract = "BACKGROUND: Amaranthus palmeri recently has been brought into the Midwestern USA as a contaminant in Conservation Reserve Program seeding mixes. Rapid species screening is required to mitigate the risk of continued species movement. RESULTS: Markers were developed for A. palmeri-specific nucleotide polymorphisms in the internal transcribed spacer of the ribosomal coding region. A quantitative polymerase chain reaction (qPCR) assay successfully identified A. palmeri from single-plant samples, simulated mixed-plant samples and seed mixtures. CONCLUSION: A qPCR assay for distinguishing A. palmeri from 12 other Amaranthus spp. was developed and validated. The assay can consistently detect a single A. palmeri seed when present in a pool of 100 total Amaranthus spp. seeds.",

keywords = "Amaranthus palmeri, Conservation Reserve Program, qPCR, species identification",

author = "Murphy, {Brent P.} and Plewa, {Diane E.} and Elizabeth Phillippi and Bissonnette, {Suzanne M.} and Tranel, {Patrick J.}",

note = "Funding Information: The USDA National Institute of Food and Agriculture (Hatch project ILLU-802-923) provided partial funding of this research. Publisher Copyright: {\textcopyright} 2017 Society of Chemical Industry",

year = "2017",

month = nov,

doi = "10.1002/ps.4632",

language = "English (US)",

volume = "73",

pages = "2221--2224",

journal = "Pest Management Science",

issn = "1526-498X",

publisher = "John Wiley & Sons, Ltd.",

number = "11",

}

TY - JOUR

T1 - A quantitative assay for Amaranthus palmeri identification

AU - Murphy, Brent P.

AU - Plewa, Diane E.

AU - Phillippi, Elizabeth

AU - Bissonnette, Suzanne M.

AU - Tranel, Patrick J.

N1 - Funding Information: The USDA National Institute of Food and Agriculture (Hatch project ILLU-802-923) provided partial funding of this research. Publisher Copyright: © 2017 Society of Chemical Industry

PY - 2017/11

Y1 - 2017/11

N2 - BACKGROUND: Amaranthus palmeri recently has been brought into the Midwestern USA as a contaminant in Conservation Reserve Program seeding mixes. Rapid species screening is required to mitigate the risk of continued species movement. RESULTS: Markers were developed for A. palmeri-specific nucleotide polymorphisms in the internal transcribed spacer of the ribosomal coding region. A quantitative polymerase chain reaction (qPCR) assay successfully identified A. palmeri from single-plant samples, simulated mixed-plant samples and seed mixtures. CONCLUSION: A qPCR assay for distinguishing A. palmeri from 12 other Amaranthus spp. was developed and validated. The assay can consistently detect a single A. palmeri seed when present in a pool of 100 total Amaranthus spp. seeds.

AB - BACKGROUND: Amaranthus palmeri recently has been brought into the Midwestern USA as a contaminant in Conservation Reserve Program seeding mixes. Rapid species screening is required to mitigate the risk of continued species movement. RESULTS: Markers were developed for A. palmeri-specific nucleotide polymorphisms in the internal transcribed spacer of the ribosomal coding region. A quantitative polymerase chain reaction (qPCR) assay successfully identified A. palmeri from single-plant samples, simulated mixed-plant samples and seed mixtures. CONCLUSION: A qPCR assay for distinguishing A. palmeri from 12 other Amaranthus spp. was developed and validated. The assay can consistently detect a single A. palmeri seed when present in a pool of 100 total Amaranthus spp. seeds.

KW - Amaranthus palmeri

KW - Conservation Reserve Program

KW - qPCR

KW - species identification

UR - http://www.scopus.com/inward/record.url?scp=85027198808&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85027198808&partnerID=8YFLogxK

U2 - 10.1002/ps.4632

DO - 10.1002/ps.4632

M3 - Article

C2 - 28580655

AN - SCOPUS:85027198808

SN - 1526-498X

VL - 73

SP - 2221

EP - 2224

JO - Pest Management Science

JF - Pest Management Science

IS - 11

ER -

A quantitative assay for Amaranthus palmeri identification

Abstract

Keywords

ASJC Scopus subject areas

Online availability

Library availability

Related links

Fingerprint

Cite this