A quantitative assay for Amaranthus palmeri identification

Brent P. Murphy, Diane E. Plewa, Elizabeth Phillippi, Suzanne M. Bissonnette, Patrick J. Tranel

Research output: Contribution to journalArticle

Abstract

BACKGROUND: Amaranthus palmeri recently has been brought into the Midwestern USA as a contaminant in Conservation Reserve Program seeding mixes. Rapid species screening is required to mitigate the risk of continued species movement. RESULTS: Markers were developed for A. palmeri-specific nucleotide polymorphisms in the internal transcribed spacer of the ribosomal coding region. A quantitative polymerase chain reaction (qPCR) assay successfully identified A. palmeri from single-plant samples, simulated mixed-plant samples and seed mixtures. CONCLUSION: A qPCR assay for distinguishing A. palmeri from 12 other Amaranthus spp. was developed and validated. The assay can consistently detect a single A. palmeri seed when present in a pool of 100 total Amaranthus spp. seeds.

Original languageEnglish (US)
Pages (from-to)2221-2224
Number of pages4
JournalPest Management Science
Volume73
Issue number11
DOIs
StatePublished - Nov 2017

Keywords

  • Amaranthus palmeri
  • Conservation Reserve Program
  • qPCR
  • species identification

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Insect Science

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    Murphy, B. P., Plewa, D. E., Phillippi, E., Bissonnette, S. M., & Tranel, P. J. (2017). A quantitative assay for Amaranthus palmeri identification. Pest Management Science, 73(11), 2221-2224. https://doi.org/10.1002/ps.4632