A Protein Kinase, Calcineurin B-Like Protein-interacting Protein Kinase9, interacts with calcium sensor Calcineurin B-Like Protein3 and regulates potassium homeostasis under low-potassium stress in Arabidopsis

Li Li Liu, Hui Min Ren, Li Qing Chen, Yi Wang, Wei Hua Wu

Research output: Contribution to journalArticlepeer-review

Abstract

Potassium (K+) is an essential macronutrient for plant growth and development. Previous studies have demonstrated that Calcineurin B-Like Protein1 (CBL1) or CBL9 and CBL-Interacting Protein Kinase23 (CIPK23) regulate K+ uptake in Arabidopsis (Arabidopsis thaliana) roots by modulating K+ channel Arabidopsis K+ Transporter1. In this study, we show that the protein kinase CIPK9 interacts with the calcium sensor CBL3 and plays crucial roles in K+ homeostasis under low-K+ stress in Arabidopsis. Arabidopsis wild-type plants showed leaf chlorotic symptoms when grown for 10 d on low-K+ (100 mM) medium. Here, we show that plants lacking CIPK9 displayed a tolerant phenotype to low-K+ stress, which still maintained green leaves when the wild-type plants showed typical K+-deficient symptoms. Overexpressing lines of CIPK9 resulted in a low-K+-sensitive phenotype compared with wild-type plants. Furthermore, CBL2 and CBL3 were identified as upstream regulators of CIPK9. Both CBL2- and CBL3-overexpressing lines displayed similar low-K+-sensitive phenotypes and K+ contents to CIPK9- overexpressing lines. However, only cbl3 mutant plants, but not cbl2 mutant plants, showed the low-K+-tolerant phenotype similar to cipk9 mutants. Taken together, these results demonstrate that CIPK9 and CBL3 work together and function in K+ homeostasis under low-K+ stress in Arabidopsis.

Original languageEnglish (US)
Pages (from-to)266-277
Number of pages12
JournalPlant physiology
Volume161
Issue number1
DOIs
StatePublished - Jan 2013
Externally publishedYes

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

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