A proposed fluorimetric determination of unsaturated iron binding capacity

D. Dlott, M. Siegel, R. Bersohn

Research output: Contribution to journalArticle

Abstract

The method is based on the discovery by C.K. Luk that the fluorescence of terbium ion (Tb3+) is enhanced enormously when it binds to an empty Fe3+ binding site of transferrin. Tb3+ cannot displace Fe3+ from an occupied binding site on transferrin. The analytic procedure is to dilute the serum (in order to lower its ultraviolet absorbance), add a solution of Tb3+, excite with ultraviolet radiation, and measure the intensity of the resulting green fluorescence. With proper calibration this intensity is a measure of UIBC. Analyses of 65 serum samples previously measured at a hospital laboratory show no obvious difficulty with the method. Its absolute accuracy has not been probed in detail.

Original languageEnglish (US)
Pages (from-to)217-224
Number of pages8
JournalAmerican journal of clinical pathology
Volume64
Issue number2
DOIs
StatePublished - Jan 1 1975

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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