TY - JOUR
T1 - A proposed fluorimetric determination of unsaturated iron binding capacity
AU - Dlott, D.
AU - Siegel, M.
AU - Bersohn, R.
PY - 1975
Y1 - 1975
N2 - The method is based on the discovery by C.K. Luk that the fluorescence of terbium ion (Tb3+) is enhanced enormously when it binds to an empty Fe3+ binding site of transferrin. Tb3+ cannot displace Fe3+ from an occupied binding site on transferrin. The analytic procedure is to dilute the serum (in order to lower its ultraviolet absorbance), add a solution of Tb3+, excite with ultraviolet radiation, and measure the intensity of the resulting green fluorescence. With proper calibration this intensity is a measure of UIBC. Analyses of 65 serum samples previously measured at a hospital laboratory show no obvious difficulty with the method. Its absolute accuracy has not been probed in detail.
AB - The method is based on the discovery by C.K. Luk that the fluorescence of terbium ion (Tb3+) is enhanced enormously when it binds to an empty Fe3+ binding site of transferrin. Tb3+ cannot displace Fe3+ from an occupied binding site on transferrin. The analytic procedure is to dilute the serum (in order to lower its ultraviolet absorbance), add a solution of Tb3+, excite with ultraviolet radiation, and measure the intensity of the resulting green fluorescence. With proper calibration this intensity is a measure of UIBC. Analyses of 65 serum samples previously measured at a hospital laboratory show no obvious difficulty with the method. Its absolute accuracy has not been probed in detail.
UR - http://www.scopus.com/inward/record.url?scp=0016798395&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0016798395&partnerID=8YFLogxK
U2 - 10.1093/ajcp/64.2.217
DO - 10.1093/ajcp/64.2.217
M3 - Article
C2 - 1155384
AN - SCOPUS:0016798395
SN - 0002-9173
VL - 64
SP - 217
EP - 224
JO - American journal of clinical pathology
JF - American journal of clinical pathology
IS - 2
ER -