@inproceedings{7c6207bbc4f6417a90bbc80c6cb60344,
title = "A preclinical small animal imaging platform combining multi-angle photoacoustic and fluorescence projections into co-registered 3D maps",
abstract = "We present the results on development of the 3D imaging platform combining photoacoustic tomography and fluorescence (PAFT) for preclinical and biological research. This combined multimodal imaging instrument addresses known deficiencies in sensitivity, spatial resolution, and anatomical registration of the individual imaging components. Multiangle photoacoustic projections, excited by an OPO operating in the near-infrared window, of a live anesthetized animal are used to reconstruct large volumes (30 cm3) that show deep anatomical vasculature and blood-rich tissues with resolutions exceeding 150 μm. A sCMOS camera is used for simultaneous co-registered multi-angle optical imaging. The images of a fluorescent dual-contrast agent are then reconstructed into a 3D volume using a tomographic algorithm. A separate 532-nm low-energy pulsed laser excitation is used for skin topography and imaging of superficial vasculature. All three imaging channels can be combined to produce spatially accurate in vivo volumes showing an animal's skin, deep anatomical structures, and distribution of photosensitive molecular contrast agents. PAFT's photoacoustic sensitivity was assessed using contrast agents in a phantom study. We demonstrate biomedical imaging application of PAFT's combined imaging modalities by observing biodistribution of a dual-contrast agent injected intravenously to in vivo preclinical murine models.",
keywords = "Fluorescence, Molecular imaging, Optical imaging, Photoacoustic tomography, Photosound, Preclinical imaging, Quantitative imaging, Small animal imaging",
author = "Weylan Thompson and Anthony Yu and Dumani, {Diego S.} and Jason Cook and Anastasio, {Mark A.} and Emelianov, {Stanislav Y.} and Ermilov, {Sergey A.}",
note = "Publisher Copyright: {\textcopyright} 2020 SPIE.; Photons Plus Ultrasound: Imaging and Sensing 2020 ; Conference date: 02-02-2020 Through 05-02-2020",
year = "2020",
doi = "10.1117/12.2549088",
language = "English (US)",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Oraevsky, {Alexander A.} and Wang, {Lihong V.}",
booktitle = "Photons Plus Ultrasound",
}