Abstract
A mammalian cell-based optical biosensor was built to detect pathogenic Listeria and Bacillus species. This sensor measures the ability of the pathogens to infect and induce cytotoxicity on hybrid lymphocyte cell line (Ped-2E9) resulting in the release of alkaline phosphatase (ALP) that can be detected optically using a portable spectrophotometer. The Ped-2E9 cells were encapsulated in collagen gel matrices and grown in 48-well plates or in specially designed filtration tube units. Toxin preparations or bacterial cells were introduced and ALP release was assayed after 3-5 h. Pathogenic L. monocytogenes strains or the listeriolysin toxins preparation showed cytotoxicity ranging from 55% 92%. Toxin preparations (∼20 πg/ml) from B. cereus strains showed 24 - 98% cytotoxicity. In contrast, a non-pathogenic L. innocua (F4247) and a B. substilis induced only 2% and 8% cytotoxicity, respectively. This cell-based detection device demonstrates its ability to detect the presence of pathogenic Listeria and Bacillus species and can potentially be used onsite for food safety or in biosecurity application.
Original language | English (US) |
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Article number | 599602 |
Journal | Proceedings of SPIE - The International Society for Optical Engineering |
Volume | 5996 |
DOIs | |
State | Published - 2005 |
Externally published | Yes |
Event | Optical Sensors and Sensing Systems for Natural Resources and Food Safety and Quality - Boston, MA, United States Duration: Oct 23 2005 → Oct 24 2005 |
Keywords
- Bacillus
- Cell-based sensor
- Collagen
- Cytotoxicity assay
- Listeria
- Optical sensor
- Ped-2E9 hybridoma cells
- Toxin
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Computer Science Applications
- Applied Mathematics
- Electrical and Electronic Engineering