TY - JOUR
T1 - A porcine macrophage cell line that supports high levels of replication of OURT88/3, an attenuated strain of African swine fever virus
AU - Portugal, Raquel
AU - Goatley, Lynnette C.
AU - Husmann, Robert
AU - Zuckermann, Federico A.
AU - Dixon, Linda K.
N1 - We acknowledge financial support from Biotechnology and Biological Sciences Research Council (BBSRC) [grant numbers BBS/E/ 1/00007031, BBS/E/1/00007034], and Department of Environment Food and Rural Affairs (DEFRA) [grant number SE1516]. Some parts of the quoted research were funded in part by the Bill & Melinda Gates Foundation and Department for international development with United Kingdom (UK) Aid from the UK Government through Global Alliance for Livestock Veterinary Medicines (GALVmed) [grant number OPP1009497]. We acknowledge financial support from BBSRC BBS/E/ 1/00007031, 7034, and DEFRA. Some parts of the quoted research were funded in part by the Bill & Melinda Gates Foundation and Department for international development with United Kingdom (UK) Aid from the UK Government through Global Alliance for Livestock Veterinary Medicines (GALVmed) (grant number OPP1009497). The findings and conclusions contained within are those of the authors and do not necessarily reflect positions or policies of the Bill & Melinda Gates Foundation or the UK Government. The funders (Bill & Melinda Gates Foundation and the UK Government) had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Assistance with animal experiments was provided from Animal Services staff at Pirbright Institute.
We acknowledge financial support from BBSRC BBS/E/ 1/00007031, 7034, and DEFRA. Some parts of the quoted research were funded in part by the Bill & Melinda Gates Foundation and Department for international development with United Kingdom (UK) Aid from the UK Government through Global Alliance for Livestock Veterinary Medicines (GALVmed) (grant number OPP1009497). The findings and conclusions contained within are those of the authors and do not necessarily reflect positions or policies of the Bill & Melinda Gates Foundation or the UK Government. The funders (Bill & Melinda Gates Foundation and the UK Government) had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Assistance with animal experiments was provided from Animal Services staff at Pirbright Institute.
PY - 2020/1/1
Y1 - 2020/1/1
N2 - The main target cells for African swine fever virus (ASFV) replication in pigs are of monocyte macrophage lineage and express markers typical of the intermediate to late stages of differentiation. The lack of a porcine cell line, which accurately represents these target cells, limits research on virus host interactions and the development of live-attenuated vaccine strains. We show here that the continuously growing, growth factor dependent ZMAC-4 porcine macrophage cell line is susceptible to infection with eight different field isolates of ASFV. Replication in ZMAC-4 cells occurred with similar kinetics and to similar high titres as in primary porcine bone marrow cells. In addition we showed that twelve passages of an attenuated strain of ASFV, OURT88/3, in ZMAC-4 cells did not reduce the ability of this virus to induce protection against challenge with virulent virus. Thus, the ZMAC-4 cells provide an alternative to primary cells for ASFV replication.
AB - The main target cells for African swine fever virus (ASFV) replication in pigs are of monocyte macrophage lineage and express markers typical of the intermediate to late stages of differentiation. The lack of a porcine cell line, which accurately represents these target cells, limits research on virus host interactions and the development of live-attenuated vaccine strains. We show here that the continuously growing, growth factor dependent ZMAC-4 porcine macrophage cell line is susceptible to infection with eight different field isolates of ASFV. Replication in ZMAC-4 cells occurred with similar kinetics and to similar high titres as in primary porcine bone marrow cells. In addition we showed that twelve passages of an attenuated strain of ASFV, OURT88/3, in ZMAC-4 cells did not reduce the ability of this virus to induce protection against challenge with virulent virus. Thus, the ZMAC-4 cells provide an alternative to primary cells for ASFV replication.
KW - African swine fever virus
KW - ZMAC
KW - macrophage cell line
KW - vaccine
KW - virus replication
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U2 - 10.1080/22221751.2020.1772675
DO - 10.1080/22221751.2020.1772675
M3 - Article
C2 - 32515659
AN - SCOPUS:85086298539
SN - 2222-1751
VL - 9
SP - 1245
EP - 1253
JO - Emerging Microbes and Infections
JF - Emerging Microbes and Infections
IS - 1
ER -