A Plastic Colorimetric Resonant Optical Biosensor for Multiparallel Detection of Label-Free Biochemical Interactions

Brian T Cunningham; Jean Qiu; Bo Lin; Peter Li; Jane Pepper

A Plastic Colorimetric Resonant Optical Biosensor for Multiparallel Detection of Label-Free Biochemical Interactions

Brian T Cunningham, Jean Qiu, Bo Lin, Peter Li, Jane Pepper

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

A high sensitivity plastic biosensor based on detection of changes in optical density on the surface of a narrow bandwidth guided mode resonant filter is demonstrated. Using sub-micron microreplication of a master sensor surface structure on continuous sheets of plastic film, the sensor can be produced inexpensively over large surface areas. In this work, the sensor structure is incorporated into standard 96-well microtiter plates and used to perform a protein-protein affinity assay. A surface receptor immobilization protocol demonstrating low nonspecific binding is used to detect an antibody with 8.3 nM sensitivity. By measuring the kinetic interaction of a protein-protein binding pair simultaneously at several concentrations, the affinity binding constant can be quickly determined.

Original language	English (US)
Title of host publication	Proceedings of IEEE Sensors
Pages	212-216
Number of pages	5
Volume	1
Edition	1
State	Published - 2002
Externally published	Yes
Event	First IEEE International Conference on Sensors - IEEE Sensors 2002 - Orlando, FL, United States Duration: Jun 12 2002 → Jun 14 2002

Other

Other	First IEEE International Conference on Sensors - IEEE Sensors 2002
Country	United States
City	Orlando, FL
Period	6/12/02 → 6/14/02

Keywords

Diagnostics
Genomics
Microreplication
Optical biosensor
Proteomics
Resonant filter

ASJC Scopus subject areas

Engineering (miscellaneous)
Electrical and Electronic Engineering

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Cite this

Cunningham, B. T., Qiu, J., Lin, B., Li, P., & Pepper, J. (2002). A Plastic Colorimetric Resonant Optical Biosensor for Multiparallel Detection of Label-Free Biochemical Interactions. In Proceedings of IEEE Sensors (1 ed., Vol. 1, pp. 212-216)

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title = "A Plastic Colorimetric Resonant Optical Biosensor for Multiparallel Detection of Label-Free Biochemical Interactions",

abstract = "A high sensitivity plastic biosensor based on detection of changes in optical density on the surface of a narrow bandwidth guided mode resonant filter is demonstrated. Using sub-micron microreplication of a master sensor surface structure on continuous sheets of plastic film, the sensor can be produced inexpensively over large surface areas. In this work, the sensor structure is incorporated into standard 96-well microtiter plates and used to perform a protein-protein affinity assay. A surface receptor immobilization protocol demonstrating low nonspecific binding is used to detect an antibody with 8.3 nM sensitivity. By measuring the kinetic interaction of a protein-protein binding pair simultaneously at several concentrations, the affinity binding constant can be quickly determined.",

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author = "Cunningham, {Brian T} and Jean Qiu and Bo Lin and Peter Li and Jane Pepper",

year = "2002",

language = "English (US)",

volume = "1",

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booktitle = "Proceedings of IEEE Sensors",

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AU - Cunningham, Brian T

AU - Qiu, Jean

AU - Lin, Bo

AU - Li, Peter

AU - Pepper, Jane

PY - 2002

Y1 - 2002

N2 - A high sensitivity plastic biosensor based on detection of changes in optical density on the surface of a narrow bandwidth guided mode resonant filter is demonstrated. Using sub-micron microreplication of a master sensor surface structure on continuous sheets of plastic film, the sensor can be produced inexpensively over large surface areas. In this work, the sensor structure is incorporated into standard 96-well microtiter plates and used to perform a protein-protein affinity assay. A surface receptor immobilization protocol demonstrating low nonspecific binding is used to detect an antibody with 8.3 nM sensitivity. By measuring the kinetic interaction of a protein-protein binding pair simultaneously at several concentrations, the affinity binding constant can be quickly determined.

AB - A high sensitivity plastic biosensor based on detection of changes in optical density on the surface of a narrow bandwidth guided mode resonant filter is demonstrated. Using sub-micron microreplication of a master sensor surface structure on continuous sheets of plastic film, the sensor can be produced inexpensively over large surface areas. In this work, the sensor structure is incorporated into standard 96-well microtiter plates and used to perform a protein-protein affinity assay. A surface receptor immobilization protocol demonstrating low nonspecific binding is used to detect an antibody with 8.3 nM sensitivity. By measuring the kinetic interaction of a protein-protein binding pair simultaneously at several concentrations, the affinity binding constant can be quickly determined.

KW - Diagnostics

KW - Genomics

KW - Microreplication

KW - Optical biosensor

KW - Proteomics

KW - Resonant filter

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Y2 - 12 June 2002 through 14 June 2002

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