A Plastic Colorimetric Resonant Optical Biosensor for Multiparallel Detection of Label-Free Biochemical Interactions

Brian T Cunningham, Jean Qiu, Bo Lin, Peter Li, Jane Pepper

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A high sensitivity plastic biosensor based on detection of changes in optical density on the surface of a narrow bandwidth guided mode resonant filter is demonstrated. Using sub-micron microreplication of a master sensor surface structure on continuous sheets of plastic film, the sensor can be produced inexpensively over large surface areas. In this work, the sensor structure is incorporated into standard 96-well microtiter plates and used to perform a protein-protein affinity assay. A surface receptor immobilization protocol demonstrating low nonspecific binding is used to detect an antibody with 8.3 nM sensitivity. By measuring the kinetic interaction of a protein-protein binding pair simultaneously at several concentrations, the affinity binding constant can be quickly determined.

Original languageEnglish (US)
Title of host publicationProceedings of IEEE Sensors
Pages212-216
Number of pages5
Volume1
Edition1
StatePublished - 2002
Externally publishedYes
EventFirst IEEE International Conference on Sensors - IEEE Sensors 2002 - Orlando, FL, United States
Duration: Jun 12 2002Jun 14 2002

Other

OtherFirst IEEE International Conference on Sensors - IEEE Sensors 2002
CountryUnited States
CityOrlando, FL
Period6/12/026/14/02

    Fingerprint

Keywords

  • Diagnostics
  • Genomics
  • Microreplication
  • Optical biosensor
  • Proteomics
  • Resonant filter

ASJC Scopus subject areas

  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering

Cite this

Cunningham, B. T., Qiu, J., Lin, B., Li, P., & Pepper, J. (2002). A Plastic Colorimetric Resonant Optical Biosensor for Multiparallel Detection of Label-Free Biochemical Interactions. In Proceedings of IEEE Sensors (1 ed., Vol. 1, pp. 212-216)