A Plant-Derived Multi-HIV Antigen Induces Broad Immune Responses in Orally Immunized Mice

Néstor Rubio-Infante, Dania O. Govea-Alonso, Andrea Romero-Maldonado, Ana Lilia García-Hernández, Damaris Ilhuicatzi-Alvarado, Jorge A. Salazar-González, Schuyler S. Korban, Sergio Rosales-Mendoza, Leticia Moreno-Fierros

Research output: Contribution to journalArticlepeer-review

Abstract

Multi-HIV, a multiepitopic protein derived from both gp120 and gp41 envelope proteins of the human immunodeficiency virus (HIV), has been proposed as a vaccine prototype capable of inducing broad immune responses, as it carries various B and T cell epitopes from several HIV strains. In this study, the immunogenic properties of a Multi-HIV expressed in tobacco chloroplasts are evaluated in test mice. BALB/c mice orally immunized with tobacco-derived Multi-HIV have elicited antibody responses, including both the V3 loop of gp120 and the ELDKWA epitope of gp41. Based on splenocyte proliferation assays, stimulation with epitopes of the C4, V3 domain of gp120, and the ELDKWA domain of gp41 elicits positive cellular responses. Furthermore, specific interferon gamma production is observed in both CD4+ and CD8+ T cells stimulated with HIV peptides. These results demonstrate that plant-derived Multi-HIV induces T helper-specific responses. Altogether, these findings illustrate the immunogenic potential of plant-derived Multi-HIV in an oral immunization scheme. The potential of this low-cost immunization approach and its implications on HIV/AIDS vaccine development are discussed.

Original languageEnglish (US)
Pages (from-to)662-674
Number of pages13
JournalMolecular Biotechnology
Volume57
Issue number7
DOIs
StatePublished - Jul 15 2015
Externally publishedYes

Keywords

  • Biofarming
  • Glycoprotein 120
  • Glycoprotein 41
  • Human immunodeficiency virus
  • Multiepitopic vaccine
  • Neutralizing antibodies

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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