A phosphorylated, disulfide-linked membrane protein in murine cytotoxic T lymphocytes

J. Lieberman, C. R. Verret, David M Kranz, S. C. Hubbard, H. Saito, D. H. Raulet, S. Tonegawa, H. N. Eisen

Research output: Contribution to journalArticle

Abstract

The previously determined sequence of the murine T-cell γ gene and its transcription in cloned T lymphocytes suggests that the polypeptide encoded by this gene is generally present in cytotoxic T cells as a 33-kDa monomer in a disulfide-bonded dimer. The γ chain is also expected to be phosphorylated because a sequence in its cytoplasmic domain is homologous to an active site for serine phosphorylation in the regulatory subunit of cAMP-dependent protein kinase. We describe here a cytotoxic T-cell-associated phosphorylated protein, many of whose properties suggest that it may be the product of the T-cell γ gene. Its phosphorylation is greatly enhanced by interleukin 2 stimulation.

Original languageEnglish (US)
Pages (from-to)7870-7874
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number20
DOIs
StatePublished - Dec 1 1986

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Cytotoxic T-Lymphocytes
Disulfides
Membrane Proteins
T-Lymphocytes
Phosphorylation
Genes
Cyclic AMP-Dependent Protein Kinases
Serine
Interleukin-2
Catalytic Domain
Peptides
Proteins

ASJC Scopus subject areas

  • General

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A phosphorylated, disulfide-linked membrane protein in murine cytotoxic T lymphocytes. / Lieberman, J.; Verret, C. R.; Kranz, David M; Hubbard, S. C.; Saito, H.; Raulet, D. H.; Tonegawa, S.; Eisen, H. N.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, No. 20, 01.12.1986, p. 7870-7874.

Research output: Contribution to journalArticle

Lieberman, J. ; Verret, C. R. ; Kranz, David M ; Hubbard, S. C. ; Saito, H. ; Raulet, D. H. ; Tonegawa, S. ; Eisen, H. N. / A phosphorylated, disulfide-linked membrane protein in murine cytotoxic T lymphocytes. In: Proceedings of the National Academy of Sciences of the United States of America. 1986 ; Vol. 83, No. 20. pp. 7870-7874.
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