A pathway for isethionate dissimilation in Bacillus krulwichiae

Yang Tong, Yifeng Wei, Yiling Hu, Ee Lui Ang, Huimin Zhao, Yan Zhang

Research output: Contribution to journalArticle

Abstract

Hydroxyethyl sulfonate (isethionate) is widely distributed in the environment as an industrial pollutant and as a product of microbial metabolism. It is used as a substrate for growth by metabolically diverse environmental bacteria. Aerobic pathways for isethionate dissimilation in Gram-negative bacteria involve the cytochrome c-dependent oxidation of isethionate to sulfoacetaldehyde by a membranebound flavoenzyme (IseJ), followed by C-S cleavage by the thiamine pyrophosphate (TPP)-dependent enzyme sulfoacetaldehyde acetyltransferase (Xsc). Here, we report a bioinformatics analysis of Xsc-containing gene clusters in Gram-positive bacteria, which revealed the presence of an alternative isethionate dissimilation pathway involving the NAD+-dependent oxidation of isethionate by a cytosolic metal-dependent alcohol dehydrogenase (IseD). We describe the biochemical characterization of recombinant IseD from the haloalkaliphilic environmental bacterium Bacillus krulwichiae AM31DT and demonstrate the growth of this bacterium using isethionate as its sole carbon source, with the excretion of sulfite as a waste product. The IseD-dependent pathway provides the only mechanism for isethionate dissimilation in Gram-positive species to date and suggests a role of the metabolically versatile Bacilli in the mineralization of this ubiquitous organosulfur compound.

Original languageEnglish (US)
Article numbere00793-19
JournalApplied and environmental microbiology
Volume85
Issue number15
DOIs
StatePublished - Jan 1 2019

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Bacillus (bacteria)
Bacillus
Bacteria
bacterium
bacteria
oxidation
Thiamine Pyrophosphate
organic sulfur compounds
Waste Products
Sulfites
acetyltransferases
Acetyltransferases
sulfonates
Alcohol Dehydrogenase
cytochrome c
sulfites
alcohol dehydrogenase
pyrophosphates
Gram-Positive Bacteria
Multigene Family

Keywords

  • Bacillus
  • Carbon source
  • Isethionate
  • Metal-dependent alcohol dehydrogenase
  • Organosulfonate
  • Sulfite
  • Taurine

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

A pathway for isethionate dissimilation in Bacillus krulwichiae. / Tong, Yang; Wei, Yifeng; Hu, Yiling; Ang, Ee Lui; Zhao, Huimin; Zhang, Yan.

In: Applied and environmental microbiology, Vol. 85, No. 15, e00793-19, 01.01.2019.

Research output: Contribution to journalArticle

Tong, Yang ; Wei, Yifeng ; Hu, Yiling ; Ang, Ee Lui ; Zhao, Huimin ; Zhang, Yan. / A pathway for isethionate dissimilation in Bacillus krulwichiae. In: Applied and environmental microbiology. 2019 ; Vol. 85, No. 15.
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