A novel way to characterize the non-specific surface adhesion of cancer cells and understand cancer metastasis

Xin Tang, Tony Cappa, Theresa Kuhlenschmidt, Mark S Kuhlenschmidt, M Taher A Saif

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Cancer deaths are mostly caused by the metastasis of the malignant cells, not by the primary tumor itself. During metastasis, cancer cells detach from the primary tumor, spread to different tissues via blood circulation or lymph system, and reattach to invade new tissues and organs. In this project, we hypothesize that cancer cells manage their invasion by changing their surface adhesivity. To study the cell surface adhesivity, a novel and versatile microelectromechanical systems (MEMS) force sensor is developed to quantify the strength of adhesion between living cancer cells and a probe. The Silicon sensors consist of a probe and 2 flexible cantilever beams, while the probe is used to contact the cancer cell and the flexible beams are used to measure the cell force response in the range from nN to uN. The spring constant of the sensor is 14 nN/ (am. Our results demonstrate that the aggressive HCT-8 cells (from human colon adenocarcinoma) show high nonspecific adhesivity when they aggregate into cell islands, and low surface non-specific adhesivity after they disassociate from the cell islands. The surface adhesivity of less aggressive Caco-2 cells (from human colon carcinoma) and normal MA 104 cell (from monkey kidney) are found to be lower than that of before-disassociation HCT-8 cells. Furthermore, the adhesion force response of cancer cells is found to show 2-slope force behavior, which is different from previous results of focal-adhesion detachment experiments. The 2-stage force bearing model is proposed to interpret the underlying mechanism.

Original languageEnglish (US)
Title of host publicationProceedings of the ASME International Mechanical Engineering Congress and Exposition 2009, IMECE 2009
PublisherAmerican Society of Mechanical Engineers (ASME)
Pages147-149
Number of pages3
ISBN (Print)9780791843840
DOIs
StatePublished - Jan 1 2010
EventASME 2009 International Mechanical Engineering Congress and Exposition, IMECE2009 - Lake Buena Vista, FL, United States
Duration: Nov 13 2009Nov 19 2009

Publication series

NameASME International Mechanical Engineering Congress and Exposition, Proceedings
Volume11

Other

OtherASME 2009 International Mechanical Engineering Congress and Exposition, IMECE2009
CountryUnited States
CityLake Buena Vista, FL
Period11/13/0911/19/09

Fingerprint

Adhesion
Cells
Tumors
Bearings (structural)
Tissue
Silicon sensors
Sensors
Hemodynamics
Cantilever beams
MEMS
Experiments

ASJC Scopus subject areas

  • Mechanical Engineering

Cite this

Tang, X., Cappa, T., Kuhlenschmidt, T., Kuhlenschmidt, M. S., & Saif, M. T. A. (2010). A novel way to characterize the non-specific surface adhesion of cancer cells and understand cancer metastasis. In Proceedings of the ASME International Mechanical Engineering Congress and Exposition 2009, IMECE 2009 (pp. 147-149). (ASME International Mechanical Engineering Congress and Exposition, Proceedings; Vol. 11). American Society of Mechanical Engineers (ASME). https://doi.org/10.1115/IMECE2009-11953

A novel way to characterize the non-specific surface adhesion of cancer cells and understand cancer metastasis. / Tang, Xin; Cappa, Tony; Kuhlenschmidt, Theresa; Kuhlenschmidt, Mark S; Saif, M Taher A.

Proceedings of the ASME International Mechanical Engineering Congress and Exposition 2009, IMECE 2009. American Society of Mechanical Engineers (ASME), 2010. p. 147-149 (ASME International Mechanical Engineering Congress and Exposition, Proceedings; Vol. 11).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Tang, X, Cappa, T, Kuhlenschmidt, T, Kuhlenschmidt, MS & Saif, MTA 2010, A novel way to characterize the non-specific surface adhesion of cancer cells and understand cancer metastasis. in Proceedings of the ASME International Mechanical Engineering Congress and Exposition 2009, IMECE 2009. ASME International Mechanical Engineering Congress and Exposition, Proceedings, vol. 11, American Society of Mechanical Engineers (ASME), pp. 147-149, ASME 2009 International Mechanical Engineering Congress and Exposition, IMECE2009, Lake Buena Vista, FL, United States, 11/13/09. https://doi.org/10.1115/IMECE2009-11953
Tang X, Cappa T, Kuhlenschmidt T, Kuhlenschmidt MS, Saif MTA. A novel way to characterize the non-specific surface adhesion of cancer cells and understand cancer metastasis. In Proceedings of the ASME International Mechanical Engineering Congress and Exposition 2009, IMECE 2009. American Society of Mechanical Engineers (ASME). 2010. p. 147-149. (ASME International Mechanical Engineering Congress and Exposition, Proceedings). https://doi.org/10.1115/IMECE2009-11953
Tang, Xin ; Cappa, Tony ; Kuhlenschmidt, Theresa ; Kuhlenschmidt, Mark S ; Saif, M Taher A. / A novel way to characterize the non-specific surface adhesion of cancer cells and understand cancer metastasis. Proceedings of the ASME International Mechanical Engineering Congress and Exposition 2009, IMECE 2009. American Society of Mechanical Engineers (ASME), 2010. pp. 147-149 (ASME International Mechanical Engineering Congress and Exposition, Proceedings).
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