TY - JOUR
T1 - A novel promoter from soybean that is active in a complex developmental pattern with and without its proximal 650 base pairs
AU - Strömvik, Martina V.
AU - Sundararaman, Vijaya P.
AU - Vodkin, Lila O.
N1 - Funding Information:
We are thankful to Dr Steve Clough and Dr Andrew Bent for providing the vacuum infiltration device and to Ms Deborah Petrik for helping in screening the cDNA library. This project was supported by the Illinois Soybean Program Operating Board.
Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1999/9
Y1 - 1999/9
N2 - We report the isolation of a novel soybean gene, Msg, which is highly expressed in developing soybean pods. The gene shows significant homology to a family of fruit- and flower-specific genes, designated the major latex protein (MLP) homologues, so far reported in only a few species and whose functions are unknown. The MLPs are more distantly related to a group of pathogenesis-related proteins (IPR or PR-10) whose functions are likewise unknown. This is the first report of a MLP homologue in a plant for which there is already an IPR-protein reported. We performed an analysis of the Msg promoter with 14 different promoter fragments ranging from 0.65 kb to 2.26 kb, fused to the uidA (GUS) gene. High transient expression was obtained with all the constructs upon particle bombardment in soybean and green bean pods. Stable Arabidopsis transformants were obtained with the Agrobacterium vacuum infiltration method. The promoter is fully active in Arabidopsis only in plants transformed with the 2.26 kb fragment promoter, expressing GUS in nectaries, nodes, short style and in guard cells of the silique, pedicel and stem but not in mature leaves. Surprisingly, the proximal 650 bp TATA-containing region cannot function on its own in Arabidopsis and can be deleted without a change in expression pattern in both Arabidopsis and soybean. Thus, tissue-specific regions of the complex Msg promoter reside in the distal 5' regions upstream of a dispensable TATA box in contrast to many examples of tissue-specific elements that reside much closer to the TATA box.
AB - We report the isolation of a novel soybean gene, Msg, which is highly expressed in developing soybean pods. The gene shows significant homology to a family of fruit- and flower-specific genes, designated the major latex protein (MLP) homologues, so far reported in only a few species and whose functions are unknown. The MLPs are more distantly related to a group of pathogenesis-related proteins (IPR or PR-10) whose functions are likewise unknown. This is the first report of a MLP homologue in a plant for which there is already an IPR-protein reported. We performed an analysis of the Msg promoter with 14 different promoter fragments ranging from 0.65 kb to 2.26 kb, fused to the uidA (GUS) gene. High transient expression was obtained with all the constructs upon particle bombardment in soybean and green bean pods. Stable Arabidopsis transformants were obtained with the Agrobacterium vacuum infiltration method. The promoter is fully active in Arabidopsis only in plants transformed with the 2.26 kb fragment promoter, expressing GUS in nectaries, nodes, short style and in guard cells of the silique, pedicel and stem but not in mature leaves. Surprisingly, the proximal 650 bp TATA-containing region cannot function on its own in Arabidopsis and can be deleted without a change in expression pattern in both Arabidopsis and soybean. Thus, tissue-specific regions of the complex Msg promoter reside in the distal 5' regions upstream of a dispensable TATA box in contrast to many examples of tissue-specific elements that reside much closer to the TATA box.
KW - Fruit-specific gene expression
KW - Glycine max
KW - Major latex protein homologue
KW - Pod development
KW - Promoter analysis
KW - Transgenic plants
UR - http://www.scopus.com/inward/record.url?scp=0344065377&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0344065377&partnerID=8YFLogxK
U2 - 10.1023/A:1006312228617
DO - 10.1023/A:1006312228617
M3 - Article
C2 - 10579489
AN - SCOPUS:0344065377
SN - 0167-4412
VL - 41
SP - 217
EP - 231
JO - Plant Molecular Biology
JF - Plant Molecular Biology
IS - 2
ER -